BMRB Entry 5928

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5928
MolProbity Validation Chart

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Title:
NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana
Deposition date:
2003-09-01
Original release date:
2004-04-07
Authors:
Lopez-Mendez, Blanca; Pantoja-Uceda, David; Tomizawa, Tadashi; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter
Citation:

Citation: Lopez-Mendez, Blanca; Pantoja-Uceda, David; Tomizawa, Tadashi; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter. "Letter to the Editor: NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana"  J. Biomol. NMR 29, 205-206 (2004).
PubMed: 15014234

Assembly members:

Assembly members:
enth-vhs domain, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
enth-vhs domain: GSSGSSGESYWRSRMIDAVT SDEDKVAPVYKLEEICDLLR SSHVSIVKEFSEFILKRLDN KSPIVKQKALRLIKYAVGKS GSEFRREMQRNSVAVRNLFH YKGHPDPLKGDALNKAVRET AHETISAIFSEENGSGPSSG

Data sets:
Data typeCount
13C chemical shifts585
15N chemical shifts129
1H chemical shifts924

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1enth-vhs monomer1

Entities:

Entity 1, enth-vhs monomer 140 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUSERTYR
2   TRPARGSERARGMETILEASPALAVALTHR
3   SERASPGLUASPLYSVALALAPROVALTYR
4   LYSLEUGLUGLUILECYSASPLEULEUARG
5   SERSERHISVALSERILEVALLYSGLUPHE
6   SERGLUPHEILELEULYSARGLEUASPASN
7   LYSSERPROILEVALLYSGLNLYSALALEU
8   ARGLEUILELYSTYRALAVALGLYLYSSER
9   GLYSERGLUPHEARGARGGLUMETGLNARG
10   ASNSERVALALAVALARGASNLEUPHEHIS
11   TYRLYSGLYHISPROASPPROLEULYSGLY
12   ASPALALEUASNLYSALAVALARGGLUTHR
13   ALAHISGLUTHRILESERALAILEPHESER
14   GLUGLUASNGLYSERGLYPROSERSERGLY

Samples:

sample_1: enth-vhs domain, [U-13C; U-15N], 1.20 mM; Tris buffer 20 mM; NaCl 100 mM; dithiothreitol 1 mM; sodium azide 0.02 % (v/v); H2O 90 % (v/v); D2O 10 % (v/v)

condition_set_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D [1H,15N ]-HSQCnot availablenot availablenot available
2D [1H,13C]-HSQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available
3D (H)CC(CO)NHnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D H(CCCO)NHnot availablenot availablenot available
3D (H)CCH-TOCSYnot availablenot availablenot available
3D 15N-edited [1H,1H]-NOESYnot availablenot availablenot available
3D 13C-edited [1H,1H]-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AV 800 MHz

Related Database Links:

PDB
DBJ BAB01267 BAH57080
GB AAB63649 AAK25955 AAK64101 AEE75792
REF NP_566540 XP_010419285 XP_010465644 XP_010465645 XP_010487480
SP Q9C5H4
AlphaFold Q9C5H4 Q9C5H4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks