BMRB Entry 5926
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5926
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Tian, F.; Valafar, H.; Prestegard, J.. "A Dipolar Coupling Based Strategy for Simultaneous Resonance Assignment and
Structure Determination of Protein Backbones" J. Am. Chem. Soc. 123, 11791-11796 (2001).
PubMed: 11716736
Assembly members:
Rubredoxin, polymer, 53 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Rubredoxin: AKYVCKICGYIYDEDAGDPD
NGVSPGTKFEEIPDDWVCPI
CGAPKSEFEKLED
| Data type | Count |
| residual dipolar couplings | 419 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rubredoxin | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, Rubredoxin 53 residues - Formula weight is not available
| 1 | ALA | LYS | TYR | VAL | CYS | LYS | ILE | CYS | GLY | TYR | ||||
| 2 | ILE | TYR | ASP | GLU | ASP | ALA | GLY | ASP | PRO | ASP | ||||
| 3 | ASN | GLY | VAL | SER | PRO | GLY | THR | LYS | PHE | GLU | ||||
| 4 | GLU | ILE | PRO | ASP | ASP | TRP | VAL | CYS | PRO | ILE | ||||
| 5 | CYS | GLY | ALA | PRO | LYS | SER | GLU | PHE | GLU | LYS | ||||
| 6 | LEU | GLU | ASP |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Rubredoxin, [U-15N], 4.5 mM; Na phosphate buffer 50 mM; NaCl 100 mM; D2O 10%; H2O 90%
sample_2: Rubredoxin, [U-15N], 2.5 mM; Na phosphate buffer 50 mM; NaCl 50 mM; 3:1 DMPC:DHPC 7%; CTAB 0.21%; D2O 10%; H2O 90%
sample_3: Rubredoxin, [U-15N], 4.5 mM; Na phosphate buffer 50 mM; NaCl 100 mM; pf1 phage mM; D2O 10%; H2O 90%
Phage_cond: pH: 6.3; temperature: 298 K
Bicelle_cond: pH: 6.3; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N coupled HSQC | not available | not available | not available |
| soft HNCA-E.COSY | not available | not available | not available |
| modified HNCO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 800 MHz