BMRB Entry 5906

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5906

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Title:
1H, 15N, and 13C resonance Assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000) -A Protein Inhibitor of Protein Phosphatase-1
Deposition date:
2003-08-18
Original release date:
2004-02-13
Authors:
Lin, Ta-Hsien
Citation:

Citation: Lin, Ta-Hsien; Huang, Yi-Choang; Chin, Mei-ling; Chen, Yi-chen; Jeng, Hao-Hsuan; Lin, Fang-Min; Shiao, Ming-Shi; Horiuchi, Atsuko; Greengard, Paul; Nairn, Angus; Huang, Hsien-bin. "Letter to Editor: 1H, 15N, and 13C resonance Assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000) - a protein inhibitor of protein phosphatase-1"  J. Biomol. NMR 28, 413-414 (2004).
PubMed: 14872138

Assembly members:

Assembly members:
dopamine and cAMP-regulated phosphoprotein, Mr. 32,000, polymer, 118 residues, 13477.48 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dopamine and cAMP-regulated phosphoprotein, Mr. 32,000: MDPKDRKKIQFSVPAPPSQL DPRQVEMIRRRRPTPALLFR VSEHSSPEEESSPHQRTSGE GHHPKSKRPNPCAYTPPSLK AVQRIAESHLQTISNLSENQ ASEEEDELGELRELGYPQ

Data sets:
Data typeCount
13C chemical shifts451
15N chemical shifts98
1H chemical shifts535

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DARPP-321

Entities:

Entity 1, DARPP-32 118 residues - 13477.48 Da.

1   METASPPROLYSASPARGLYSLYSILEGLN
2   PHESERVALPROALAPROPROSERGLNLEU
3   ASPPROARGGLNVALGLUMETILEARGARG
4   ARGARGPROTHRPROALALEULEUPHEARG
5   VALSERGLUHISSERSERPROGLUGLUGLU
6   SERSERPROHISGLNARGTHRSERGLYGLU
7   GLYHISHISPROLYSSERLYSARGPROASN
8   PROCYSALATYRTHRPROPROSERLEULYS
9   ALAVALGLNARGILEALAGLUSERHISLEU
10   GLNTHRILESERASNLEUSERGLUASNGLN
11   ALASERGLUGLUGLUASPGLULEUGLYGLU
12   LEUARGGLULEUGLYTYRPROGLN

Samples:

sample_1: dopamine and cAMP-regulated phosphoprotein, Mr. 32,000, [U-99% 13C; U-99% 15N], 1 mM

Ex-cond_1: pH: 5.5; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

BMRB 15176 15865
GB AAH78954 AAT11858 EDM05918 EDM05919
REF NP_612530
SP Q6J4I0
AlphaFold Q6J4I0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks