BMRB Entry 5905

Title:
Backbone 15N and 1HN Chemical Shift Assignments for Sac7d
Deposition date:
2003-08-18
Original release date:
2007-07-11
Authors:
Clark, Andrew
Citation:

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile proteins Sac7d and Sso7d"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
Sac7d, polymer, 66 residues, 7609 Da.

Natural source:

Natural source:   Common Name: Sulfolobus acidocaldarius Brock et al   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus acidocaldarius

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts67
13C chemical shifts13
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sac7d monomer1

Entities:

Entity 1, Sac7d monomer 66 residues - 7609 Da.

1   METVALLYSVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLUVALASPTHRSERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETVAL
4   SERPHETHRTYRASPASPASNGLYLYSTHR
5   GLYARGGLYALAVALSERGLULYSASPALA
6   PROLYSGLULEULEUASPMETLEUALAARG
7   ALAGLUARGGLULYSLYS

Samples:

sample: Sac7d, [U-98% 15N], 1.2 mM; KCl 0.3 M; H2O 90%; D2O 10%; DSS 100 uM

conditions: pH: 4.07 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCsamplenot availableconditions
3D HSQC-TOCSYsamplenot availableconditions
3D HSQC-NOESYsamplenot availableconditions

Software:

NMRPipe v5 - data processing, transformation, referencing

NMRView - sequential assignments

NMR spectrometers:

  • Varian VXR-S 500 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5908
PDB
GB AAA80315 AAY79492 AGE70041 AGE72316 AHC50588
REF WP_011276993 YP_007433189 YP_007435464 YP_254785
SP P13123
AlphaFold P13123 P13123 P13123 P13123 P13123 P13123

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks