BMRB Entry 5901
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5901
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Berkovits-Cymet, H.; Amann, B.; Berg, J.. "Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1 and its
Implications for DNA Binding " Biochemistry 43, 898-903 (2004).
PubMed: 14744132
Assembly members:
neural zinc finger transcription factor 1, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: 10116 Taxonomy ID: Norway rat Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNZF-1E
Entity Sequences (FASTA):
neural zinc finger transcription factor 1: MHVKKPYYDPSRTEKRESKC
PTPGCDGTGHVTGLYPHHRS
LSGCPHKDRVPPEILAMHEN
VLK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 216 |
| 15N chemical shifts | 40 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ZINC BINDING DOMAIN | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, ZINC BINDING DOMAIN 63 residues - Formula weight is not available
| 1 | MET | HIS | VAL | LYS | LYS | PRO | TYR | TYR | ASP | PRO | ||||
| 2 | SER | ARG | THR | GLU | LYS | ARG | GLU | SER | LYS | CYS | ||||
| 3 | PRO | THR | PRO | GLY | CYS | ASP | GLY | THR | GLY | HIS | ||||
| 4 | VAL | THR | GLY | LEU | TYR | PRO | HIS | HIS | ARG | SER | ||||
| 5 | LEU | SER | GLY | CYS | PRO | HIS | LYS | ASP | ARG | VAL | ||||
| 6 | PRO | PRO | GLU | ILE | LEU | ALA | MET | HIS | GLU | ASN | ||||
| 7 | VAL | LEU | LYS |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: neural zinc finger transcription factor 1 2.3 mM; ZnCl2 2.3 mM; deuterated Tris 55 mM
sample_2: neural zinc finger transcription factor 1, [U-15N], 2.8 mM; ZnCl2 2.8 mM; deuterated Tris 36 mM; H2O 98%; D2O 2%
sample_3: neural zinc finger transcription factor 1, [U-15N], 1.5 mM; CdCl2 1.5 mM; deuterated Tris 36 mM
sample_cond_1: pH: 6.95; temperature: 293 K; pressure: 1 atm
sample_cond_2: pH: 7.02; temperature: 303 K; pressure: 1 atm
sample_cond_3: pH: 7.00; temperature: 303 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 15N HSQC | not available | not available | not available |
| 15N HSQC LongRange | not available | not available | not available |
| 3D 15N HSQC-TOCSY | not available | not available | not available |
| 3D 15N HSQC-NOESY | not available | not available | not available |
Software:
VNMR v6.1b - collection
FELIX v98 - processing
In house perl scripts - data analysis
CNS v1.1 - structure solution, refinement
NMR spectrometers:
- Varian UNITYplus 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks