BMRB Entry 5898

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5898

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignments for the 24.4 kDa human gankyrin protein

Deposition date:

2003-08-05

Original release date:

2004-10-25

Authors:

Yuan, Chunhua; Li, Junan; Poi, Mingjye; Byeon, In-Ja; Tsai, Ming-Daw

Citation:

Citation: Yuan, Chunhua; Li, Junan; Mahajan, A.; Poi, Mingjye; Byeon, In-Ja; Tsai, Ming-Daw. "Solution structure of the human oncogenic protein gankyrin containing seven ankyrin repeats and analysis of its structure--function relationship." Biochemistry 43, 12152-12161 (2004).
PubMed: 15379554

Assembly members:

Assembly members:
gankyrin, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gankyrin: MEGCVSNLMVCNLAYSGKLE ELKESILADKSLATRTDQDS RTALHWACSAGHTEIVEFLL QLGVPVNDKDDAGWSPLHIA ASAGRDEIVKALLGKGAQVN AVNQNGCTPLHYAASKNRHE IAVMLLEGGANPDAKDHYEA TAMHRAAAKGNLKMIHILLY YKASTNIQDTEGNTPLHLAC DEERVEEAKLLVSQGASIYI ENKEEKTPLQVAKGGLGLIL KRMVEG

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	428
13C chemical shifts	642
15N chemical shifts	217

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gankyrin	1

Entities:

Entity 1, gankyrin 226 residues - Formula weight is not available

1

MET

GLU

GLY

CYS

VAL

SER

ASN

LEU

MET

VAL

2

CYS

ASN

LEU

ALA

TYR

SER

GLY

LYS

LEU

GLU

3

GLU

LEU

LYS

GLU

SER

ILE

LEU

ALA

ASP

LYS

4

SER

LEU

ALA

THR

ARG

THR

ASP

GLN

ASP

SER

5

ARG

THR

ALA

LEU

HIS

TRP

ALA

CYS

SER

ALA

6

GLY

HIS

THR

GLU

ILE

VAL

GLU

PHE

LEU

7

GLN

LEU

GLY

VAL

PRO

VAL

ASN

ASP

LYS

ASP

8

ASP

ALA

GLY

TRP

SER

PRO

LEU

HIS

ILE

ALA

9

ALA

SER

ALA

GLY

ARG

ASP

GLU

ILE

VAL

LYS

10

ALA

LEU

GLY

LYS

GLY

ALA

GLN

VAL

ASN

11

ALA

VAL

ASN

GLN

ASN

GLY

CYS

THR

PRO

LEU

12

HIS

TYR

ALA

SER

LYS

ASN

ARG

HIS

GLU

13

ILE

ALA

VAL

MET

LEU

GLU

GLY

ALA

14

ASN

PRO

ASP

ALA

LYS

ASP

HIS

TYR

GLU

ALA

15

THR

ALA

MET

HIS

ARG

ALA

LYS

GLY

16

ASN

LEU

LYS

MET

ILE

HIS

ILE

LEU

TYR

17

TYR

LYS

ALA

SER

THR

ASN

ILE

GLN

ASP

THR

18

GLU

GLY

ASN

THR

PRO

LEU

HIS

LEU

ALA

CYS

19

ASP

GLU

ARG

VAL

GLU

ALA

LYS

LEU

20

LEU

VAL

SER

GLN

GLY

ALA

SER

ILE

TYR

ILE

21

GLU

ASN

LYS

GLU

LYS

THR

PRO

LEU

GLN

22

VAL

ALA

LYS

GLY

LEU

GLY

LEU

ILE

LEU

23

LYS

ARG

MET

VAL

GLU

GLY

Samples:

sample_1: gankyrin, [U-13C; U-15N; U-70% 2H], 0.4 – 0.5 mM; HEPES 5 mM; EDTA 1 uM; DTT 1 mM

Ex-cond_1: pH: 7.4; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
TROSY-HNCA	not available	not available	not available
TROSY-HN(CO)CA	not available	not available	not available
TROSY-HNCACB	not available	not available	not available
TROSY-HNCO	not available	not available	not available
15N-edited NOESY-HSQC	not available	not available	not available

Software:

XWINNMR v3.1 -

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DRX 800 MHz

PDB	1QYM 1TR4 1UOH 4NIK
DBJ	BAA33215 BAA34594 BAE87161 BAE87972 BAE88401
GB	AAH11960 AAV38495 AAX41449 AAY18907 ACT64478
REF	NP_001248034 NP_002805 XP_001926942 XP_002763200 XP_002832022
SP	O75832
AlphaFold	O75832