BMRB Entry 5894

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5894

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C, and 15N resonance assignments for the N-terminal domain of Drosophila Stem-Loop Binding Protein

Deposition date:

2003-07-31

Original release date:

2004-10-25

Authors:

Thapar, Roopa; Marzluff, William

Citation:

Citation: Thapar, Roopa; Mueller, G.; Marzluff, William. "The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structure." Biochemistry 43, 9390-9400 (2004).
PubMed: 15260482

Assembly members:

Assembly members:
Stem loop binding protein, polymer, 113 residues, 12070 Da.

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Stem loop binding protein: MGSSHHHHHHSSGLVPRGSH MGSGSLNSSASSISIDVKPT MQSWAQEVRAEFGHSDEASS SLNSSAASCGSLAKKETADG NLESKDGEGREMAFEFLDGV NEVKFERLVKEEK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	82
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Drosophila SLBP N-terminus	1

Entities:

Entity 1, Drosophila SLBP N-terminus 113 residues - 12070 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLY

SER

GLY

SER

LEU

ASN

SER

ALA

4

SER

ILE

SER

ILE

ASP

VAL

LYS

PRO

THR

5

MET

GLN

SER

TRP

ALA

GLN

GLU

VAL

ARG

ALA

6

GLU

PHE

GLY

HIS

SER

ASP

GLU

ALA

SER

7

SER

LEU

ASN

SER

ALA

SER

CYS

GLY

8

SER

LEU

ALA

LYS

GLU

THR

ALA

ASP

GLY

9

ASN

LEU

GLU

SER

LYS

ASP

GLY

GLU

GLY

ARG

10

GLU

MET

ALA

PHE

GLU

PHE

LEU

ASP

GLY

VAL

11

ASN

GLU

VAL

LYS

PHE

GLU

ARG

LEU

VAL

LYS

12

GLU

LYS

Samples:

Sample_1: Stem loop binding protein, [U-15N; U-13C], 2 mM

condition_1: ionic strength: 0.050 M; pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
(15N,1H) HSQC	Sample_1	not available	condition_1
HNCA	Sample_1	not available	condition_1
HN(CO)CA	Sample_1	not available	condition_1
HNCO	Sample_1	not available	condition_1
HNCACB	Sample_1	not available	condition_1
CC(CO)NH TOCSY	Sample_1	not available	condition_1
HCCH-TOCSY	Sample_1	not available	condition_1
HNHA	Sample_1	not available	condition_1
1H-15N NOESY	Sample_1	not available	condition_1

Software:

FELIX - data analysis, data processing

NMR spectrometers:

Varian INOVA 600 MHz

GB	AAF56867 AAF71752 AAG16723 AAL90413 AAM50696
REF	NP_477480
SP	Q9VAN6
AlphaFold	Q9VAN6