BMRB Entry 5893

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5893

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Unusual Ca2+-calmodulin binding interactions of the microtubule-associated protein F-STOP

Deposition date:

2003-07-31

Original release date:

2004-03-05

Authors:

Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Gans, Pierre

Citation:

Citation: Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Arlaud, Gerard; Bally, Isabelle; Forge, Vincent; Margolis, Robert; Gans, Pierre; Kleman, Jean-Philippe. "Unusual Ca(2+)-calmodulin Binding Interactions of the Microtubule-associated Protein F-STOP" Biochemistry 42, 11484-11493 (2003).
PubMed: 14516200

Assembly members:

Assembly members:
calmodulin, polymer, 163 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: MSHHHHHHSMDIEFMADQLT DEQIAEFKEAFSLFDKDGDG TITTKELGTVMRSLGQNPTE AELQDMINEVDADGNGTIDF PEFLNLMARKMKDTDSEEEL KEAFRVFDKDGNGFISAAEL RHVMTNLGEKLTDEEVDEMI READVDGDGQVNYEEFVQVM MAK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	152
13C chemical shifts	301
15N chemical shifts	152

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	calmodulin peptide	1
2	CALCIUM (II) ION	2

Entities:

Entity 1, calmodulin peptide 163 residues - Formula weight is not available

1

MET

SER

HIS

SER

MET

2

ASP

ILE

GLU

PHE

MET

ALA

ASP

GLN

LEU

THR

3

ASP

GLU

GLN

ILE

ALA

GLU

PHE

LYS

GLU

ALA

4

PHE

SER

LEU

PHE

ASP

LYS

ASP

GLY

ASP

GLY

5

THR

ILE

THR

LYS

GLU

LEU

GLY

THR

VAL

6

MET

ARG

SER

LEU

GLY

GLN

ASN

PRO

THR

GLU

7

ALA

GLU

LEU

GLN

ASP

MET

ILE

ASN

GLU

VAL

8

ASP

ALA

ASP

GLY

ASN

GLY

THR

ILE

ASP

PHE

9

PRO

GLU

PHE

LEU

ASN

LEU

MET

ALA

ARG

LYS

10

MET

LYS

ASP

THR

ASP

SER

GLU

LEU

11

LYS

GLU

ALA

PHE

ARG

VAL

PHE

ASP

LYS

ASP

12

GLY

ASN

GLY

PHE

ILE

SER

ALA

GLU

LEU

13

ARG

HIS

VAL

MET

THR

ASN

LEU

GLY

GLU

LYS

14

LEU

THR

ASP

GLU

VAL

ASP

GLU

MET

ILE

15

ARG

GLU

ALA

ASP

VAL

ASP

GLY

ASP

GLY

GLN

16

VAL

ASN

TYR

GLU

PHE

VAL

GLN

VAL

MET

17

MET

ALA

LYS

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: calmodulin, [U-97% 13C; U-98% 15N], 0.7 mM

Condition_1: pH: 7.7; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	Condition_1
HNCA	sample_1	not available	Condition_1
HNCO	sample_1	not available	Condition_1
HN(CO)CA	sample_1	not available	Condition_1
(H)CC(CO)NH	sample_1	not available	Condition_1

Software:

FELIX v2000 - raw spectral data processing, peak picking

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 800 MHz

PDB	1QS7 1QTX 1VRK
DBJ	BAJ85642 BAJ99455
EMBL	CAA46150 CAA74307
GB	AAA72492 AAA72766 AAC61858 AAC61859 AAD10246
REF	NP_001105490 XP_004961667 XP_007146213