BMRB Entry 5891
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5891
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kahsai, Mebrahtu; Singh, Sanjay; Gupta, Ramesh; Edmondson, Stephen; Shriver, John. "1H, 15N and 13C NMR Assignments and Secondary Structure of a Hyperthermophile
DNA-binding Protein Predicted to contain an Extended Coiled Coil Domain" J. Biomol. NMR ., .-. (2003).
Assembly members:
Sso10a, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sso10a: AKKKSKLEIIQAILEACKSG
SPKTRIMYGANLSYALTGRY
IKMLMDLEIIRQEGKQYMLT
KKGEELLEDIRKFNEMRKNM
DQLKEKINSVLSIRQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 644 |
| 13C chemical shifts | 443 |
| 15N chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso10a subunit 1 | 1 |
| 2 | Sso10a subunit 2 | 1 |
Entities:
Entity 1, Sso10a subunit 1 95 residues - Formula weight is not available
| 1 | ALA | LYS | LYS | LYS | SER | LYS | LEU | GLU | ILE | ILE | ||||
| 2 | GLN | ALA | ILE | LEU | GLU | ALA | CYS | LYS | SER | GLY | ||||
| 3 | SER | PRO | LYS | THR | ARG | ILE | MET | TYR | GLY | ALA | ||||
| 4 | ASN | LEU | SER | TYR | ALA | LEU | THR | GLY | ARG | TYR | ||||
| 5 | ILE | LYS | MET | LEU | MET | ASP | LEU | GLU | ILE | ILE | ||||
| 6 | ARG | GLN | GLU | GLY | LYS | GLN | TYR | MET | LEU | THR | ||||
| 7 | LYS | LYS | GLY | GLU | GLU | LEU | LEU | GLU | ASP | ILE | ||||
| 8 | ARG | LYS | PHE | ASN | GLU | MET | ARG | LYS | ASN | MET | ||||
| 9 | ASP | GLN | LEU | LYS | GLU | LYS | ILE | ASN | SER | VAL | ||||
| 10 | LEU | SER | ILE | ARG | GLN |
Samples:
sample_1: Sso10a, [U-98% 13C; U-98% 15N], 2.5 mM
Condition_1: pH: 5.0 na; temperature: 303 K; ionic strength: 0.0 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNHA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NNH | not available | not available | not available |
| NOESY-NHSQC | not available | not available | not available |
| CCC-TOCSY-NNH | not available | not available | not available |
| HCC-TOCY-NNH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HBCBCGCDHD | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
| 2D-dq-COSY | not available | not available | not available |
Software:
NMRVIEW v5 - Back bone and side chain assignment
NMPIPE - NMR data processing
FELIX - NMR data processing
VNMR - data collection, data processing
NMR spectrometers:
- Varian INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks