BMRB Entry 5888

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5888

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Title:
Backbone 1H, 15N, CA and CB assignments for the W7A mutant of rat nuclear transport factor 2 (NTF2)
Deposition date:
2003-07-30
Original release date:
2003-12-19
Authors:
Morrison, Jonathan; Yang, Ji-Chun; Stewart, Murray; Neuhaus, David
Citation:

Citation: Morrison, Jonathan; Yang, Ji-Chun; Stewart, Murray; Neuhaus, David. "Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats"  J. Mol. Biol. 333, 587-603 (2003).
PubMed: 14556747

Assembly members:

Assembly members:
Nuclear transport factor 2 W7A mutant, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nuclear transport factor 2 W7A mutant: MGDKPIAEQIGSSFIQHYYQ LFDNDRTQLGAIYIDASCLT WEGQQFQGKAAIVEKLSSLP FQKIQHSITAQDHQPTPDSC IISMVVGQLKADEDPIMGFH QMFLLKNINDAWVCTNDMFR LALHNFG

Data sets:
Data typeCount
1H chemical shifts136
13C chemical shifts237
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTF2 W7A subunit 11
2NTF2 W7A subunit 21

Entities:

Entity 1, NTF2 W7A subunit 1 127 residues - Formula weight is not available

1   METGLYASPLYSPROILEALAGLUGLNILE
2   GLYSERSERPHEILEGLNHISTYRTYRGLN
3   LEUPHEASPASNASPARGTHRGLNLEUGLY
4   ALAILETYRILEASPALASERCYSLEUTHR
5   TRPGLUGLYGLNGLNPHEGLNGLYLYSALA
6   ALAILEVALGLULYSLEUSERSERLEUPRO
7   PHEGLNLYSILEGLNHISSERILETHRALA
8   GLNASPHISGLNPROTHRPROASPSERCYS
9   ILEILESERMETVALVALGLYGLNLEULYS
10   ALAASPGLUASPPROILEMETGLYPHEHIS
11   GLNMETPHELEULEULYSASNILEASNASP
12   ALATRPVALCYSTHRASNASPMETPHEARG
13   LEUALALEUHISASNPHEGLY

Samples:

sample_1: Nuclear transport factor 2 W7A mutant0.4 – 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: Nuclear transport factor 2 W7A mutant, [U-98% 15N], 0.4 – 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_3: Nuclear transport factor 2 W7A mutant, [U-98% 13C; U-98% 15N], 0.4 – 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

Ex-cond_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 15N-HSQCnot availablenot availablenot available
2D 13C-HSQCnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNHAHBnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available

Software:

xwin-nmr v2.6 - processing

sparky v3.91 - visualisation during assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 5887
PDB
DBJ BAB22117 BAB28283 BAB32122 BAC25936 BAC34511
EMBL CAA30278 CAA62839 CAG33218 CAH91946
GB AAA85905 AAH02348 AAH03955 AAH61569 AAH83165
REF NP_001007630 NP_001032713 NP_001126129 NP_001271650 NP_005787
SP P61970 P61971 P61972 Q32KP9 Q5R8G4
TPG DAA20137
AlphaFold P61970 P61971 P61972 Q32KP9 Q5R8G4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks