BMRB Entry 5880

Name: Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22
Published: 2006-01-11

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PDB ID: 1uhu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5880
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22

Deposition date:

2003-07-23

Original release date:

2006-01-11

Authors:

Suzuki, S.; Hatanaka, H.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.

Citation:

Citation: Suzuki, S.; Hatanaka, H.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.. "Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22" .

Assembly members:

Assembly members:
RIKEN cDNA 3110009E22, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RIKEN cDNA 3110009E22: GSSGSSGTPLSLTLDHWSEI RSRAHNLSVEIKKGPWRTFC ASEWPTFDVGWPPEGTFDLT VIFEVKAIVFQDGPGSHPDQ QPYITVWQDLVQNSPPWIKS GPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	452
15N chemical shifts	108
1H chemical shifts	675

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	product of RIKEN cDNA 3110009E22	1

Entities:

Entity 1, product of RIKEN cDNA 3110009E22 105 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

PRO

LEU

2

SER

LEU

THR

LEU

ASP

HIS

TRP

SER

GLU

ILE

3

ARG

SER

ARG

ALA

HIS

ASN

LEU

SER

VAL

GLU

4

ILE

LYS

GLY

PRO

TRP

ARG

THR

PHE

CYS

5

ALA

SER

GLU

TRP

PRO

THR

PHE

ASP

VAL

GLY

6

TRP

PRO

GLU

GLY

THR

PHE

ASP

LEU

THR

7

VAL

ILE

PHE

GLU

VAL

LYS

ALA

ILE

VAL

PHE

8

GLN

ASP

GLY

PRO

GLY

SER

HIS

PRO

ASP

GLN

9

GLN

PRO

TYR

ILE

THR

VAL

TRP

GLN

ASP

LEU

10

VAL

GLN

ASN

SER

PRO

TRP

ILE

LYS

SER

11

GLY

PRO

SER

GLY

Samples:

sample_1: RIKEN cDNA 3110009E22, [U-13C; U-15N], 3.2 mM; phosphate buffer Na 20 mM; NaCl 100 mM; d-DTT 1 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0.12 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

VNMR v6.1B - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.771 - data analysis

Olivia v1.9.5 - data analysis

CYANA v1.0.7 - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz