BMRB Entry 5873

Click here to enlarge.

PDB ID: 1p6r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5873
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone and side chains 1H, 13C, and 15N Chemical Shift Assignments for BlaI-NTD

Deposition date:

2003-07-18

Original release date:

2004-07-07

Authors:

Van Melckebeke, Helene; Vreuls, Christelle; Gans, Pierre; Filee, Patrice; Llabres, Gabriel; Joris, Bernard; Simorre, Jean-Pierre

Citation:

Citation: Van Melckebeke, Helene; Vreuls, Christelle; Gans, Pierre; Filee, Patrice; Llabres, Gabriel; Joris, Bernard; Simorre, Jean-Pierre. "Solution Structural Study of BlaI : Implications for the Repression of Genes Involved in Beta-lactam Antibiotic Resistance" J. Mol. Biol. 333, 711-720 (2003).
PubMed: 14568532

Assembly members:

Assembly members:
b-lactamase gene repressor, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus licheniformis Taxonomy ID: 1402 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus licheniformis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
b-lactamase gene repressor: MKKIPQISDAELEVMKVIWK HSSINTNEVIKELSKTSTWS PKTIQTMLLRLIKKGALNHH KEGRVFVYTPNIDESDYIEV KS

Data sets:

assigned_chemical_shifts
- shift_set1

Data type	Count
1H chemical shifts	513
13C chemical shifts	351
15N chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BlaI-NTD	1

Entities:

Entity 1, BlaI-NTD 82 residues - Formula weight is not available

1

MET

LYS

ILE

PRO

GLN

ILE

SER

ASP

ALA

2

GLU

LEU

GLU

VAL

MET

LYS

VAL

ILE

TRP

LYS

3

HIS

SER

ILE

ASN

THR

ASN

GLU

VAL

ILE

4

LYS

GLU

LEU

SER

LYS

THR

SER

THR

TRP

SER

5

PRO

LYS

THR

ILE

GLN

THR

MET

LEU

ARG

6

LEU

ILE

LYS

GLY

ALA

LEU

ASN

HIS

7

LYS

GLU

GLY

ARG

VAL

PHE

VAL

TYR

THR

PRO

8

ASN

ILE

ASP

GLU

SER

ASP

TYR

ILE

GLU

VAL

9

LYS

SER

Samples:

sample_1: b-lactamase gene repressor, [U-13C; U-15N], 0.5 mM; phosphate buffer 75 mM; KCl 300 mM; H2O 90%; D2O 10%

sample_2: b-lactamase gene repressor, [U-15N], 0.75 mM

set_condition_1: pH: 7.6 na; temperature: 298 K; ionic strength: 375 mM

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
CBCANH	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
(H)C(CO)NH-TOCSY	not available	not available	not available
H(CCO)NH-TOCSY	not available	not available	not available
1H-15N HSQC	not available	not available	not available
H(C)CH-TOCSY	not available	not available	not available
2D-NOESY	not available	not available	not available

Software:

FELIX v2000 -

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

PDB	1P6R 2P7C
GB	AAA22648 AAA22650 AAU21886 AAU39238 AGN34711
REF	WP_003178582 WP_020449991 WP_026585720 YP_006711714 YP_008076448
SP	P06555
AlphaFold	P06555 P06555