BMRB Entry 5867

Title:
Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)
Deposition date:
2003-07-14
Original release date:
2006-01-11
Authors:
Suzuki, S.; Hatanaka, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation:

Citation: Suzuki, S.; Hatanaka, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)"  .

Assembly members:

Assembly members:
INTERSECTIN 2, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts67
1H chemical shifts392

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1INTERSECTIN 21

Entities:

Entity 1, INTERSECTIN 2 69 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYGLUGLU
2   TYRILEALALEUTYRPROTYRSERSERVAL
3   GLUPROGLYASPLEUTHRPHETHRGLUGLY
4   GLUGLUILELEUVALTHRGLNLYSASPGLY
5   GLUTRPTRPTHRGLYSERILEGLYASPARG
6   SERGLYILEPHEPROSERASNTYRVALLYS
7   PROLYSASPSERGLYPROSERSERGLY

Samples:

sample_1: INTERSECTIN 2, [U-13C; U-15N], 6.6 mM; phosphate buffer Na 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0.12 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

xwinnmr v2.6 - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.771 - data analysis

Olivia v1.9.5 - data analysis

CYANA v1.0.7 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts