BMRB Entry 5865
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5865
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Zhang, Naixia; Li, Minghua; Chen, Xiang; Wang, Yuefeng; Wu, Gong; Hu, Guoyuan; Wu, Houming. "Solution structure of BmKK2, a new potassium channel blocker from the venom of
chinese scorpion Buthus martensi Karsch" Proteins 55, 835-845 (2004).
PubMed: 15146482
Assembly members:
K+ toxin-like peptide, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: Chinese scorpion Taxonomy ID: 34649 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Buthus martensii Karsch
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
K+ toxin-like peptide: TPFAIKCATDADCSRKCPGN
PPCRNGFCACT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 191 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | K+ toxin-like peptide | 1 |
Entities:
Entity 1, K+ toxin-like peptide 31 residues - Formula weight is not available
| 1 | THR | PRO | PHE | ALA | ILE | LYS | CYS | ALA | THR | ASP | ||||
| 2 | ALA | ASP | CYS | SER | ARG | LYS | CYS | PRO | GLY | ASN | ||||
| 3 | PRO | PRO | CYS | ARG | ASN | GLY | PHE | CYS | ALA | CYS | ||||
| 4 | THR |
Samples:
sample_1: K+ toxin-like peptide 2.7 mM; H2O 90%; D2O 10%
sample_2: K+ toxin-like peptide 2.7 mM; D2O 100%
sample_cond_1: pH: 3.0; pressure: 1 atm; temperature: 300 K
sample_cond_2: pH: 2.6; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
VNMR v6.1B - collection, processing
XEASY v1994 - data analysis
DYANA v1.5 - structure solution
AMBER v5.0 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz