BMRB Entry 5861
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5861
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Monleon, Daniel; Yim, Lucia; Martinez-Vicente, Marta; Armengod, M.; Celda, Bernardo. "Letter to the Editor: Backbone 1H, 13C and 15N resonance assignments for the 18.7 kDa
GTPase domain of Escherichia coli MnmE protein" J. Biomol. NMR 28, 307-308 (2004).
PubMed: 14752268
Assembly members:
gtpase domain mnme, polymer, 172 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
gtpase domain mnme: GSLLREGMKVVIAGRPNAGK
SSLLNALAGREAAIVTDIAG
TTRDVLREHIHIDGMPLHII
DTAGLREASDEVERIGIERA
WQEIEQADRVLFMVDGTTTD
AVDPAEIWPEFIARLPAKLP
ITVVRNKADITGETLGMSEV
NGHALIRLSARTGEGVDVLR
NHLKQSMGIHRD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 329 |
| 13C chemical shifts | 462 |
| 15N chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gtpase domain MnmE | 1 |
Entities:
Entity 1, gtpase domain MnmE 172 residues - Formula weight is not available
| 1 | GLY | SER | LEU | LEU | ARG | GLU | GLY | MET | LYS | VAL | ||||
| 2 | VAL | ILE | ALA | GLY | ARG | PRO | ASN | ALA | GLY | LYS | ||||
| 3 | SER | SER | LEU | LEU | ASN | ALA | LEU | ALA | GLY | ARG | ||||
| 4 | GLU | ALA | ALA | ILE | VAL | THR | ASP | ILE | ALA | GLY | ||||
| 5 | THR | THR | ARG | ASP | VAL | LEU | ARG | GLU | HIS | ILE | ||||
| 6 | HIS | ILE | ASP | GLY | MET | PRO | LEU | HIS | ILE | ILE | ||||
| 7 | ASP | THR | ALA | GLY | LEU | ARG | GLU | ALA | SER | ASP | ||||
| 8 | GLU | VAL | GLU | ARG | ILE | GLY | ILE | GLU | ARG | ALA | ||||
| 9 | TRP | GLN | GLU | ILE | GLU | GLN | ALA | ASP | ARG | VAL | ||||
| 10 | LEU | PHE | MET | VAL | ASP | GLY | THR | THR | THR | ASP | ||||
| 11 | ALA | VAL | ASP | PRO | ALA | GLU | ILE | TRP | PRO | GLU | ||||
| 12 | PHE | ILE | ALA | ARG | LEU | PRO | ALA | LYS | LEU | PRO | ||||
| 13 | ILE | THR | VAL | VAL | ARG | ASN | LYS | ALA | ASP | ILE | ||||
| 14 | THR | GLY | GLU | THR | LEU | GLY | MET | SER | GLU | VAL | ||||
| 15 | ASN | GLY | HIS | ALA | LEU | ILE | ARG | LEU | SER | ALA | ||||
| 16 | ARG | THR | GLY | GLU | GLY | VAL | ASP | VAL | LEU | ARG | ||||
| 17 | ASN | HIS | LEU | LYS | GLN | SER | MET | GLY | ILE | HIS | ||||
| 18 | ARG | ASP |
Samples:
sample_1: gtpase domain mnme, [U-95% 13C; U-95% 15N], 0.2 – 0.4 mM
sample_cond_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
Software:
Xwinnmr v3.1 - data processing
Sparky v3.104 - spectra analysis, peak picking, manual resonance assignments
AutoAssign v1.9.0 - automatic resonances pre-assignment
NMR spectrometers:
- AVANCE DRX-AVANCE 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38064 BAE77587 BAG79516 BAI28034 BAI33153 |
| EMBL | CAP78166 CAQ34051 CAQ91437 CAR00681 CAR05336 |
| GB | AAA62057 AAC76729 AAG58903 AAN45201 AAN83061 |
| REF | NP_290339 NP_312668 NP_418162 NP_709494 NP_756487 |
| SP | A1AHP0 A7ZTR2 A8A6G8 B1IX32 B1LL33 |
| AlphaFold | A1AHP0 A7ZTR2 A8A6G8 B1IX32 B1LL33 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks