BMRB Entry 5851

Name: Solution Stucture of the Third Zinc Finger from BKLF
Published: 2003-09-12

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PDB ID: 1p7a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5851
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Stucture of the Third Zinc Finger from BKLF

Deposition date:

2003-06-26

Original release date:

2003-09-12

Authors:

Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.

Citation:

Citation: Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.. "CCHX Zinc Finger Derivatives Retain the Ability to Bind Zn(II) and Mediate Protein-DNA Interactions" J. Biol. Chem. 278, 28011-28018 (2003).
PubMed: 12736264

Assembly members:

Assembly members:
Kruppel-like factor 3, polymer, 37 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Kruppel-like factor 3: GSTRGSTGIKPFQCPDCDRS FSRSDHLALHRKRHMLV

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- coupling_constants_set_1

Data type	Count
15N chemical shifts	33
1H chemical shifts	213
coupling constants	28

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Kruppel-like factor 3	1
2	ZINC ION	2

Entities:

Entity 1, Kruppel-like factor 3 37 residues - Formula weight is not available

1

GLY

SER

THR

ARG

GLY

SER

THR

GLY

ILE

LYS

2

PRO

PHE

GLN

CYS

PRO

ASP

CYS

ASP

ARG

SER

3

PHE

SER

ARG

SER

ASP

HIS

LEU

ALA

LEU

HIS

4

ARG

LYS

ARG

HIS

MET

LEU

VAL

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Kruppel-like factor 3 3 mM; TCEP 4.5 mM; ZnSO4 4.5 mM; H2O 90%; D2O 10%

sample_2: Kruppel-like factor 3, [U-15N], 1 mM; TCEP 1.5 mM; ZnSO4 1.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 280 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
2D TOCSY	not available	not available	sample_cond_1
DQF-COSY	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1

Software:

xwinnmr v2.5 - processing

DYANA v1.5 -

XEASY v1.3.13 - data analysis

ARIA v1.1.2 - refinement, structure solution

NMR spectrometers:

Bruker DRX 600 MHz