BMRB Entry 5847

Name: Backbone and sidechain 1H, 13C, and 15N chemical shifts for Sda
Published: 2003-11-21

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PDB ID: 1pv0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5847
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and sidechain 1H, 13C, and 15N chemical shifts for Sda

Deposition date:

2003-06-26

Original release date:

2003-11-21

Authors:

Rowland, Susan; Maciejewski, Mark; King, Glenn

Citation:

Citation: Rowland, Susan; Burkholder, William; Cunningham, K; Maciejewski, Mark; Grossman, Alan; King, Glenn. "Structure and mechanism of action of Sda, an inhibitor of the histidine kinases that regulate initiation of sporulation in Bacillus subtilis" Mol. Cell. 13, 689-701 (2004).
PubMed: 15023339

Assembly members:

Assembly members:
Sda, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sda: MRKLSDELLIESYFKATEMN LNRDFIELIENEIKRRSLGH IISVSS

Data sets:

assigned_chemical_shifts
- Sda_shifts

Data type	Count
1H chemical shifts	353
13C chemical shifts	201
15N chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sda	1

Entities:

Entity 1, Sda 46 residues - Formula weight is not available

1

MET

ARG

LYS

LEU

SER

ASP

GLU

LEU

ILE

2

GLU

SER

TYR

PHE

LYS

ALA

THR

GLU

MET

ASN

3

LEU

ASN

ARG

ASP

PHE

ILE

GLU

LEU

ILE

GLU

4

ASN

GLU

ILE

LYS

ARG

SER

LEU

GLY

HIS

5

ILE

SER

VAL

SER

Samples:

Sample_1: Sda, [U-13C; U-15N], 1.0 mM

cond_H2O: pH: 6.85 na; temperature: 298 K; ionic strength: 0.125 M

cond_D2O: pH: 6.85 na; temperature: 298 K; ionic strength: 0.125 mM

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HC(CO)NH-TOCSY	not available	not available	not available
3D C(CO)NH-TOCSY	not available	not available	not available
3D HNCO	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNHB	not available	not available	not available
3D HCCH-COSY	not available	not available	not available
2D 1H-1H TOCSY	not available	not available	not available
2D 1H-1H DQF-COSY	not available	not available	not available
3D 15N-separated NOESY-HSQC	not available	not available	not available
3D 13C-separated NOESY-HSQC	not available	not available	not available
3D 15N-separated TOCSY-HSQC	not available	not available	not available

Software:

NMRpipe v1.0 - NMR data processing

XEASY v1.3.13 - Spectral analysis, peak assignment

NMR spectrometers:

INOVA Varian 500 MHz
INOVA Varian 600 MHz

PDB	1PV0 3FYR
DBJ	BAI86094 GAK78421
EMBL	CAE01463 CCU59089
GB	ADM38587 ADP33164 ADV93314 AEP87448 AEP91604
REF	WP_003226124 WP_003325380 WP_010886571 YP_003866896 YP_003974095
SP	Q7WY62
AlphaFold	Q7WY62