BMRB Entry 5821

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PDB ID: 1jmc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5821
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A

Deposition date:

2003-06-06

Original release date:

2004-02-11

Authors:

Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter

Citation:

Citation: Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter. "Letter to the Editor: 1H, 13C and 15N assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A" J. Biomol. NMR 28, 195-196 (2004).
PubMed: 14755166

Assembly members:

Assembly members:
single chain biopolymer, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: 9606 Taxonomy ID: Human Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
single chain biopolymer: QSKVVPIASLTPYQSKWTIC ARVTNKSQIRTWSNSRGEGK LFSLELVDESGEIRATAFNE QVDKFFPLIEVNKVYYFSKG TLKIANKQFTAVKNDYEMTF NNETSVMPCED

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	498
15N chemical shifts	116
1H chemical shifts	786

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RPA70(181-291)	1

Entities:

Entity 1, RPA70(181-291) 111 residues - Formula weight is not available

1

GLN

SER

LYS

VAL

PRO

ILE

ALA

SER

LEU

2

THR

PRO

TYR

GLN

SER

LYS

TRP

THR

ILE

CYS

3

ALA

ARG

VAL

THR

ASN

LYS

SER

GLN

ILE

ARG

4

THR

TRP

SER

ASN

SER

ARG

GLY

GLU

GLY

LYS

5

LEU

PHE

SER

LEU

GLU

LEU

VAL

ASP

GLU

SER

6

GLY

GLU

ILE

ARG

ALA

THR

ALA

PHE

ASN

GLU

7

GLN

VAL

ASP

LYS

PHE

PRO

LEU

ILE

GLU

8

VAL

ASN

LYS

VAL

TYR

PHE

SER

LYS

GLY

9

THR

LEU

LYS

ILE

ALA

ASN

LYS

GLN

PHE

THR

10

ALA

VAL

LYS

ASN

ASP

TYR

GLU

MET

THR

PHE

11

ASN

GLU

THR

SER

VAL

MET

PRO

CYS

GLU

12

ASP

Samples:

Sample_1: single chain biopolymer, [U-15N; U-13C], 0.5 – 1.0 mM; tris-d11 20 mM; Potassium Chloride 50 mM; Magnesium Chloride 10 mM; DTT 2 mM; Sodium Azide 0.01%; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0.080 M; pH: 7.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCANH	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
H(CCO)NH-TOCSY	not available	not available	not available
(H)C(CO)NH-TOCSY	not available	not available	not available

Software:

FELIX v2000 -

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

BMRB	5823
PDB	1FGU 1JMC
DBJ	BAD92969 BAF82393 BAG10883
EMBL	CAH92206
GenBank	AAA36584 AAH18126 AAS94324 EAW90574
REF	NP_002936 XP_511254
SWISS-PROT	P27694 Q5R7Q4