BMRB Entry 5818
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5818
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Volpon, L.; Lievre, C.; Osborne, M.; Gandhi, S.; Iannuzzi, P.; Larocque, R.; Cygler, M.; Gehring, K.; Ekiel, I.. "The Solution Structure of YbcJ from Escherichia coli Reveals a Recently
Discovered alphaL Motif Involved in RNA Binding " J. Bacteriol. 185, 4204-4210 (2003).
PubMed: 12837795
Assembly members:
YbcJ, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
YbcJ: GSMIHRMSNMATFSLGKHPH
VELCDLLKLEGWSESGAQAK
IAIAEGQVKVDGAVETRKRC
KIVAGQTVSFAGHSVQVVA
- heteronucl_NOEs
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YbcJ | 1 |
Entities:
Entity 1, YbcJ 79 residues - Formula weight is not available
| 1 | GLY | SER | MET | ILE | HIS | ARG | MET | SER | ASN | MET | ||||
| 2 | ALA | THR | PHE | SER | LEU | GLY | LYS | HIS | PRO | HIS | ||||
| 3 | VAL | GLU | LEU | CYS | ASP | LEU | LEU | LYS | LEU | GLU | ||||
| 4 | GLY | TRP | SER | GLU | SER | GLY | ALA | GLN | ALA | LYS | ||||
| 5 | ILE | ALA | ILE | ALA | GLU | GLY | GLN | VAL | LYS | VAL | ||||
| 6 | ASP | GLY | ALA | VAL | GLU | THR | ARG | LYS | ARG | CYS | ||||
| 7 | LYS | ILE | VAL | ALA | GLY | GLN | THR | VAL | SER | PHE | ||||
| 8 | ALA | GLY | HIS | SER | VAL | GLN | VAL | VAL | ALA |
Samples:
sample_1: YbcJ, [U-13C; U-15N], 4 – 7 mM; phosphate buffer 50 mM; NaCl 300 mM; DTT 15 mM; sodium azide 1 mM; H2O 90%; D2O 10%
sample_2: YbcJ, [U-13C; U-15N], 4 – 7 mM; phosphate buffer 50 mM; NaCl 300 mM; DTT 15 mM; sodium azide 1 mM; Pf1 phage 8 mg/mL; H2O 90%; D2O 10%
sample_cond_1: pH: 6.8; temperature: 313 K; ionic strength: 350 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| IPAP-HSQ | not available | not available | sample_cond_1 |
Software:
GIFA v4.31 - processing
XWINNMR v2.1 - collection
XEASY v1.3.13 - data analysis
TALOS v2003.027.13.05 - refinement
ARIA v1.1 - refinement
CNS v1.1 - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz