BMRB Entry 5817

Name: NMR Structure of a Prototype LNR Module from Human Notch1
Published: 2003-09-08

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PDB ID: 1pb5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5817
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of a Prototype LNR Module from Human Notch1

Deposition date:

2003-06-04

Original release date:

2003-09-08

Authors:

Vardar, D.; North, C.; Sanchez-Irizarry, C.; Aster, J.; Blacklow, S.

Citation:

Citation: Vardar, D.; North, C.; Sanchez-Irizarry, C.; Aster, J.; Blacklow, S.. "Nuclear Magnetic Resonance Structure of a Prototype Lin12-Notch Repeat Module from Human Notch1" Biochemistry 42, 7061-7067 (2003).
PubMed: 12795601

Assembly members:

Assembly members:
Neurogenic locus notch homolog protein 1, polymer, 35 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neurogenic locus notch homolog protein 1: EEACELPECQEDAGNKVCSL QCNNHACGWDGGDCS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	82
15N chemical shifts	40
1H chemical shifts	177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Intersectin 2	1
2	CALCIUM ION	2

Entities:

Entity 1, Intersectin 2 35 residues - Formula weight is not available

1

GLU

ALA

CYS

GLU

LEU

PRO

GLU

CYS

GLN

2

GLU

ASP

ALA

GLY

ASN

LYS

VAL

CYS

SER

LEU

3

GLN

CYS

ASN

HIS

ALA

CYS

GLY

TRP

ASP

4

GLY

ASP

CYS

SER

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: Neurogenic locus notch homolog protein 1, [U-15N], 1 mM; calcium salt 10 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_2: Neurogenic locus notch homolog protein 1, [U-13C; U-15N], 1 mM; calcium salt 10 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_3: Neurogenic locus notch homolog protein 1, [U-15N], 1.5 mM; calcium salt 10 mM; DSS 0.5 mM; PIPES, [U-2H], 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_cond_2: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HNCOCA	not available	not available	not available
13C HSQC	not available	not available	not available
3D 15N-separated TOCSY	not available	not available	not available
2D TOCSY	not available	not available	not available
15N-HSQC	not available	not available	not available
15N-HMQC-J	not available	not available	not available

Software:

XWINNMR - collection

NMRPipe - processing

NmrView v5.0.4 - data analysis

CNS v1.1 - structure refinement, structure solution

NMR spectrometers:

Varian UNITY 400 MHz

PDB	1PB5
DBJ	BAD93110
EMBL	CAI13934 CAI16149
GenBank	AAA60614 AAG33848 EAW88241
REF	NP_060087 XP_001116824 XP_001171581 XP_520371
SWISS-PROT	P46531