BMRB Entry 5814

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5814

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Title:
The C-terminal domain of Viral IAP associated factor (cVIAF) is a structural homolog of phosducin: Resonance assignments and secondary structure of the C-terminal domain of VIAF
Deposition date:
2003-06-03
Original release date:
2004-02-11
Authors:
Jacob, Jaison; Louis, John; Richter, Bettina; Duckett, Colin; Torchia, Dennis
Citation:

Citation: Jacob, Jaison; Louis, John; Richter, Bettina; Duckett, Colin; Torchia, Dennis. "Letter to the Editor: The C-terminal domain of Viral IAP associated factor (cVIAF) is a structural homologue of phosducin: Resonance assignments and secondary structure of the C-terminal domain of VIAF"  J. Biomol. NMR 28, 197-198 (2004).
PubMed: 14755167

Assembly members:

Assembly members:
viral IAP associated factor, polymer, 152 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
viral IAP associated factor: KNKFGEVLEISGKDYVQEVT KAGEGLWVILHLYKQGIPLC ALINQHLSGLARKFPDVKFI KAISTTCIPNYPDRNLPTIF VYLEGDIKAQFIGPLVFGGM NLTRDELEWKLSESGAIMTD LEENPKKPIEDVLLSSVRRS VLMKRDSDSEGD

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts130
1H chemical shifts836

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cVIAF monomer1

Entities:

Entity 1, cVIAF monomer 152 residues - Formula weight is not available

1   LYSASNLYSPHEGLYGLUVALLEUGLUILE
2   SERGLYLYSASPTYRVALGLNGLUVALTHR
3   LYSALAGLYGLUGLYLEUTRPVALILELEU
4   HISLEUTYRLYSGLNGLYILEPROLEUCYS
5   ALALEUILEASNGLNHISLEUSERGLYLEU
6   ALAARGLYSPHEPROASPVALLYSPHEILE
7   LYSALAILESERTHRTHRCYSILEPROASN
8   TYRPROASPARGASNLEUPROTHRILEPHE
9   VALTYRLEUGLUGLYASPILELYSALAGLN
10   PHEILEGLYPROLEUVALPHEGLYGLYMET
11   ASNLEUTHRARGASPGLULEUGLUTRPLYS
12   LEUSERGLUSERGLYALAILEMETTHRASP
13   LEUGLUGLUASNPROLYSLYSPROILEGLU
14   ASPVALLEULEUSERSERVALARGARGSER
15   VALLEUMETLYSARGASPSERASPSERGLU
16   GLYASP

Samples:

sample_1: viral IAP associated factor, [U-90% 15N], 1.1 mM; sodium phosphate 20 mM; DTT, [U-2H], 2 mM

sample_2: viral IAP associated factor, [U-95% 13C; U-90% 15N], 1 mM

sample_3: viral IAP associated factor, [U-10% 13C; U-90% 15N], 0.8 mM

cond-1: ionic strength: 0.03 M; pH: 7.2; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HCACOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCONHnot availablenot availablenot available
CCONHnot availablenot availablenot available
3D - HCCH NOESYnot availablenot availablenot available
3D - 1H-15N NOESYnot availablenot availablenot available
13C-1H CT-HSQCnot availablenot availablenot available

Software:

NMRPipe - procesing

PIPP - analysis

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 750 MHz

Related Database Links:

DBJ BAG36697 BAH13356
EMBL CAH90983
GB AAG21887 AAG44722 AAH01021 AAX93227 ADQ33173
REF NP_001125567 NP_076970 XP_003274901 XP_003815816 XP_525834
SP Q5RB77 Q9H2J4
AlphaFold Q5RB77 Q9H2J4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks