BMRB Entry 5795
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5795
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Citation: Huang, Ren-Huai; Xiang, Y.; Tu, G.; Zhang, Y.; Wang, D.. "Solution structure of Eucommia antifungal peptide: a novel structural
model distinct with a five-disulfide motif." Biochemistry 43, 6005-6012 (2004).
PubMed: 15147184
Assembly members:
Eucommia Antifungal Peptide 2, polymer, 41 residues, 4158.77 Da.
Natural source: Common Name: Hardy Rubber Tree Taxonomy ID: 4392 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Eucommia ulmoides
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Eucommia Antifungal Peptide 2: XTCASRCPRPCNAGLCCSIY
GYCGSGAAYCGAGNCRCQCR
G
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 602 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EAFP2 monomer | 1 |
Entities:
Entity 1, EAFP2 monomer 41 residues - 4158.77 Da.
| 1 | PCA | THR | CYS | ALA | SER | ARG | CYS | PRO | ARG | PRO | ||||
| 2 | CYS | ASN | ALA | GLY | LEU | CYS | CYS | SER | ILE | TYR | ||||
| 3 | GLY | TYR | CYS | GLY | SER | GLY | ALA | ALA | TYR | CYS | ||||
| 4 | GLY | ALA | GLY | ASN | CYS | ARG | CYS | GLN | CYS | ARG | ||||
| 5 | GLY |
Samples:
sample_1: Eucommia Antifungal Peptide 25.8 – 6.2 mM
sample_2: Eucommia Antifungal Peptide 25.8 – 6.2 mM; H2O 90%; D2O 10%
sample_3: Eucommia Antifungal Peptide 25.8 – 6.2 mM; D2O 100%
Ex-Cond_1: pH: 5.6 na; temperature: 300 K
Ex-Cond_2: pH: 5.6 na; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
Software:
SPARKY v3.105 - spectra view, peak assignments, peak integrations.
NMR spectrometers:
- Bruker DMX 500 MHz