BMRB Entry 5793
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5793
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Honda, Masayoshi; Rajesh, Sundaresan; Nietlispach, Daniel; Mikawa, Tsutomu; Shibata, Takehiko; Ito, Yutaka. "Letter to the Editor: Backbone 1H, 13C, 15N assignments of a 42 kDa RecR homodimer" J. Biomol. NMR 28, 199-200 (2004).
Assembly members:
Thermus thermophilus HB8 RecR, polymer, 194 residues, 21192.5 Da.
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Thermus thermophilus HB8 RecR: MRYPESLLKLTRALSRLPGI
GPKTAQRLALHLAFHKEEAE
ALAEALEGIKRVRACRECGN
LAEGELCPICQDEDRDRSLL
AVVESVADLYALERSGEFRG
LYHVLGGALNPLEGIGPKEL
NLEGLFRRLEGVEEVVLATS
MTVEGEATALYLAEELKKRG
VRVTRPAYGLPVGGSLEYAD
EVTLGRALEGRRPV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 549 |
| 15N chemical shifts | 177 |
| 1H chemical shifts | 177 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RecR subunit 1 | 1 |
| 2 | RecR subunit 2 | 1 |
Entities:
Entity 1, RecR subunit 1 194 residues - 21192.5 Da.
| 1 | MET | ARG | TYR | PRO | GLU | SER | LEU | LEU | LYS | LEU | ||||
| 2 | THR | ARG | ALA | LEU | SER | ARG | LEU | PRO | GLY | ILE | ||||
| 3 | GLY | PRO | LYS | THR | ALA | GLN | ARG | LEU | ALA | LEU | ||||
| 4 | HIS | LEU | ALA | PHE | HIS | LYS | GLU | GLU | ALA | GLU | ||||
| 5 | ALA | LEU | ALA | GLU | ALA | LEU | GLU | GLY | ILE | LYS | ||||
| 6 | ARG | VAL | ARG | ALA | CYS | ARG | GLU | CYS | GLY | ASN | ||||
| 7 | LEU | ALA | GLU | GLY | GLU | LEU | CYS | PRO | ILE | CYS | ||||
| 8 | GLN | ASP | GLU | ASP | ARG | ASP | ARG | SER | LEU | LEU | ||||
| 9 | ALA | VAL | VAL | GLU | SER | VAL | ALA | ASP | LEU | TYR | ||||
| 10 | ALA | LEU | GLU | ARG | SER | GLY | GLU | PHE | ARG | GLY | ||||
| 11 | LEU | TYR | HIS | VAL | LEU | GLY | GLY | ALA | LEU | ASN | ||||
| 12 | PRO | LEU | GLU | GLY | ILE | GLY | PRO | LYS | GLU | LEU | ||||
| 13 | ASN | LEU | GLU | GLY | LEU | PHE | ARG | ARG | LEU | GLU | ||||
| 14 | GLY | VAL | GLU | GLU | VAL | VAL | LEU | ALA | THR | SER | ||||
| 15 | MET | THR | VAL | GLU | GLY | GLU | ALA | THR | ALA | LEU | ||||
| 16 | TYR | LEU | ALA | GLU | GLU | LEU | LYS | LYS | ARG | GLY | ||||
| 17 | VAL | ARG | VAL | THR | ARG | PRO | ALA | TYR | GLY | LEU | ||||
| 18 | PRO | VAL | GLY | GLY | SER | LEU | GLU | TYR | ALA | ASP | ||||
| 19 | GLU | VAL | THR | LEU | GLY | ARG | ALA | LEU | GLU | GLY | ||||
| 20 | ARG | ARG | PRO | VAL |
Samples:
sample_1: Thermus thermophilus HB8 RecR, [U-98% 2H; U-98% 13C; U-98% 15N], 0.6 mM
cond_set_1: pH: 7.2 na; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HN(CA)CB | not available | not available | not available |
| 3D HN(COCA)CB | not available | not available | not available |
| 3D HN(CA)CO | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
Software:
AZARA v2.7 - data processing
ANSIG for OpenGL v1.0.5 - peak assignments
NMR spectrometers:
- BRUKER DRX 600 MHz
Related Database Links:
| DBJ | BAD71423 |
| GB | AAS81578 AEG34015 AFH38354 EIA38760 |
| REF | WP_008633233 WP_011228798 WP_014629118 YP_005205 YP_005641142 |
| SP | Q5SHY0 Q72I96 |
| AlphaFold | Q5SHY0 Q72I96 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks