BMRB Entry 5789
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5789
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NMR-STAR v3 text file.
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Citation: Li, Xia; Peterkofsky, Alan; Wang, Guangshun. "Letter to the Editor: 1H,15N, and 13C Chemical Shift Assignments of the
Escherichia coli Nitrogen Regulatory Phosphocarrier IIA(Ntr)" J. Biomol. NMR 27, 401-402 (2003).
PubMed: 14512741
Assembly members:
nitrogen regulatory enzyme IIA, polymer, 163 residues, 18000 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRE-His-Tag
Entity Sequences (FASTA):
nitrogen regulatory enzyme IIA: MTNNDTTLQLSSVLNRECTR
SRVHCQSKKRALEIISELAA
KQLSLPPQVVFEAILTREKM
GSTGIGNGIAIPHGKLEEDT
LRAVGVFVQLETPIAFDAID
NQPVDLLFALLVPADQTKTH
LHTLSLVAKRLADKTICRRL
RAAQSDEELYQIITDTEGTP
DEA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 924 |
| 13C chemical shifts | 653 |
| 15N chemical shifts | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IIANtr | 1 |
Entities:
Entity 1, IIANtr 163 residues - 18000 Da.
| 1 | MET | THR | ASN | ASN | ASP | THR | THR | LEU | GLN | LEU | ||||
| 2 | SER | SER | VAL | LEU | ASN | ARG | GLU | CYS | THR | ARG | ||||
| 3 | SER | ARG | VAL | HIS | CYS | GLN | SER | LYS | LYS | ARG | ||||
| 4 | ALA | LEU | GLU | ILE | ILE | SER | GLU | LEU | ALA | ALA | ||||
| 5 | LYS | GLN | LEU | SER | LEU | PRO | PRO | GLN | VAL | VAL | ||||
| 6 | PHE | GLU | ALA | ILE | LEU | THR | ARG | GLU | LYS | MET | ||||
| 7 | GLY | SER | THR | GLY | ILE | GLY | ASN | GLY | ILE | ALA | ||||
| 8 | ILE | PRO | HIS | GLY | LYS | LEU | GLU | GLU | ASP | THR | ||||
| 9 | LEU | ARG | ALA | VAL | GLY | VAL | PHE | VAL | GLN | LEU | ||||
| 10 | GLU | THR | PRO | ILE | ALA | PHE | ASP | ALA | ILE | ASP | ||||
| 11 | ASN | GLN | PRO | VAL | ASP | LEU | LEU | PHE | ALA | LEU | ||||
| 12 | LEU | VAL | PRO | ALA | ASP | GLN | THR | LYS | THR | HIS | ||||
| 13 | LEU | HIS | THR | LEU | SER | LEU | VAL | ALA | LYS | ARG | ||||
| 14 | LEU | ALA | ASP | LYS | THR | ILE | CYS | ARG | ARG | LEU | ||||
| 15 | ARG | ALA | ALA | GLN | SER | ASP | GLU | GLU | LEU | TYR | ||||
| 16 | GLN | ILE | ILE | THR | ASP | THR | GLU | GLY | THR | PRO | ||||
| 17 | ASP | GLU | ALA |
Samples:
sample_1: nitrogen regulatory enzyme IIA, [U-95% 13C; U-95% 15N], 1.0 mM
sample_cond_1: pH: 7.3; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCA | not available | not available | not available |
| CA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
Software:
NMRPipe, PIPP - data processing, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA02317 BAB37506 BAE77248 BAG79012 BAI27482 |
| EMBL | CAA81619 CAP77664 CAQ33537 CAR00166 CAR04814 |
| GB | AAA58006 AAB60165 AAC76236 AAG58338 AAN44710 |
| REF | NP_289778 NP_312110 NP_417671 NP_709003 NP_755830 |
| SP | P69815 P69829 |
| AlphaFold | P69815 P69829 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks