BMRB Entry 5788
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5788
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Citation: Freedman, Steven; Sun, Zhen-Yu; Kung, Andrew; France, Dennis; Wagner, Gerhard; Eck, Michael. "Structural Basis for Negative Regulation of Hypoxia-inducible
Factor-1alpha by CITED2" Nat. Struct. Biol. 10, 504-512 (2003).
PubMed: 12778114
Assembly members:
CITED2 transactivation domain, polymer, 52 residues, Formula weight is not available
p300 cysteine/histidine-rich 1 domain, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CITED2 transactivation domain: GSGSGSGSNVIDTDFIDEEV
LMSLVIEMGLDRIKELPELW
LGQNEFDFMTDF
p300 cysteine/histidine-rich 1 domain: MGSGAHTADPEKRKLIQQQL
VLLLHAHKCQRREQANGEVR
QCNLPHCRTMKNVLNHMTHC
QSGKSCQVAHCASSRQIISH
WKNCTRHDCPVCLPLKNAGD
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 855 |
| 13C chemical shifts | 546 |
| 15N chemical shifts | 131 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CITED2 | 1 |
| 2 | p300 cysteine histidine-rich 1 domain | 2 |
Entities:
Entity 1, CITED2 52 residues - Formula weight is not available
| 1 | GLY | SER | GLY | SER | GLY | SER | GLY | SER | ASN | VAL | ||||
| 2 | ILE | ASP | THR | ASP | PHE | ILE | ASP | GLU | GLU | VAL | ||||
| 3 | LEU | MET | SER | LEU | VAL | ILE | GLU | MET | GLY | LEU | ||||
| 4 | ASP | ARG | ILE | LYS | GLU | LEU | PRO | GLU | LEU | TRP | ||||
| 5 | LEU | GLY | GLN | ASN | GLU | PHE | ASP | PHE | MET | THR | ||||
| 6 | ASP | PHE |
Entity 2, p300 cysteine histidine-rich 1 domain 101 residues - Formula weight is not available
| 1 | MET | GLY | SER | GLY | ALA | HIS | THR | ALA | ASP | PRO | ||||
| 2 | GLU | LYS | ARG | LYS | LEU | ILE | GLN | GLN | GLN | LEU | ||||
| 3 | VAL | LEU | LEU | LEU | HIS | ALA | HIS | LYS | CYS | GLN | ||||
| 4 | ARG | ARG | GLU | GLN | ALA | ASN | GLY | GLU | VAL | ARG | ||||
| 5 | GLN | CYS | ASN | LEU | PRO | HIS | CYS | ARG | THR | MET | ||||
| 6 | LYS | ASN | VAL | LEU | ASN | HIS | MET | THR | HIS | CYS | ||||
| 7 | GLN | SER | GLY | LYS | SER | CYS | GLN | VAL | ALA | HIS | ||||
| 8 | CYS | ALA | SER | SER | ARG | GLN | ILE | ILE | SER | HIS | ||||
| 9 | TRP | LYS | ASN | CYS | THR | ARG | HIS | ASP | CYS | PRO | ||||
| 10 | VAL | CYS | LEU | PRO | LEU | LYS | ASN | ALA | GLY | ASP | ||||
| 11 | LYS |
Samples:
sample_1: CITED2 transactivation domain, [U-15N; U-13C], 1.0 mM; p300_CH1, [U-15N; U-13C], 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM
sample_2: CITED2 transactivation domain, [U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-15N], 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM
sample_3: CITED2 transactivation domain 1.0 mM; p300 cysteine/histidine-rich 1 domain 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM
cond_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CCONH | not available | not available | not available |
| HCCONH | not available | not available | not available |
| 1H-15N-TOCSY | not available | not available | not available |
| 1H-13C-HCCH-TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 1H-1H 2D NOESY | not available | not available | not available |
Software:
PROSA - NMR processing
XEASY - peak analysis
NMR spectrometers:
- Varian UnityInova 500 MHz
- Varian UnityInova 750 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks