BMRB Entry 5787
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5787
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lou, Yuan-Chao; Pan, Yun-Ru; Ho, Yi-Hsuan; Liao, You-Di; Chen, Chinpan. "Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary
structure of the cytotoxic protein RNase 3 from oocytes of bullfrog Rana
catesbeiana" J. Biomol. NMR 27, 289-290 (2003).
Assembly members:
RC-RNase 3, polymer, 105 residues, 11876 Da.
PCA, non-polymer, 129.114 Da.
Natural source: Common Name: bullfrog Taxonomy ID: 8400 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana catesbeiana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11d
Entity Sequences (FASTA):
RC-RNase 3: XDWETFQKKHLTDTKKVKCD
VEMAKALFDCKKTNTFIYAL
PGRVKALCKNIRDNTDVLSR
DAFLLPQCDRIKLPCHYKLS
SSTNTICITCVNQLPIHFAG
VGSCP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 754 |
| 13C chemical shifts | 484 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RC-RNase 3 | 1 |
Entities:
Entity 1, RC-RNase 3 105 residues - 11876 Da.
| 1 | PCA | ASP | TRP | GLU | THR | PHE | GLN | LYS | LYS | HIS | ||||
| 2 | LEU | THR | ASP | THR | LYS | LYS | VAL | LYS | CYS | ASP | ||||
| 3 | VAL | GLU | MET | ALA | LYS | ALA | LEU | PHE | ASP | CYS | ||||
| 4 | LYS | LYS | THR | ASN | THR | PHE | ILE | TYR | ALA | LEU | ||||
| 5 | PRO | GLY | ARG | VAL | LYS | ALA | LEU | CYS | LYS | ASN | ||||
| 6 | ILE | ARG | ASP | ASN | THR | ASP | VAL | LEU | SER | ARG | ||||
| 7 | ASP | ALA | PHE | LEU | LEU | PRO | GLN | CYS | ASP | ARG | ||||
| 8 | ILE | LYS | LEU | PRO | CYS | HIS | TYR | LYS | LEU | SER | ||||
| 9 | SER | SER | THR | ASN | THR | ILE | CYS | ILE | THR | CYS | ||||
| 10 | VAL | ASN | GLN | LEU | PRO | ILE | HIS | PHE | ALA | GLY | ||||
| 11 | VAL | GLY | SER | CYS | PRO |
Samples:
sample_1: RC-RNase 3, [U-13C; U-15N], 1.0 mM
sample_2: RC-RNase 3 1.5 mM
sample_condition: pH: 3.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | sample_condition |
| 2D DQF-COSY | not available | not available | sample_condition |
| 2D NOESY | not available | not available | sample_condition |
| 2D 1H-13C HSQC | not available | not available | sample_condition |
| 2D 1H-15N HSQC | not available | not available | sample_condition |
| 3D HNCACB | not available | not available | sample_condition |
| 3D CBCA(CO)NH | not available | not available | sample_condition |
| 3D HNCO | not available | not available | sample_condition |
| 3D HN(CA)CO | not available | not available | sample_condition |
| 3D 15N NOESY-HSQC | not available | not available | sample_condition |
| 3D 15N TOCSY-HSQC | not available | not available | sample_condition |
| 3D C(CO)NH | not available | not available | sample_condition |
| 3D HC(CO)NH | not available | not available | sample_condition |
| 3D HCCH-TOCSY | not available | not available | sample_condition |
Software:
Xwinnmr v2.6 - data processing
Aurelia v2.1.3 - assignment
Nmrpipe v2.1 - data processing
Nmrview v5.0 - assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks