BMRB Entry 5780
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5780
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Liepinsh, Edvards; Barbals, Raitis; Dahl, Edgar; Sharipo, Anatoly; Staub, Eike; Otting, Gottfried. "The Death-domain Fold of the ASC PYRIN Domain, Presenting a Basis for
PYRIN/PYRIN Recognition " J. Mol. Biol. 332, 1155-1163 (2003).
PubMed: 14499617
Assembly members:
PYRIN domain, polymer, 103 residues, 10076 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PYRIN domain: MRGSHHHHHHGSMGRARDAI
LDALENLTAEELKKFKLKLL
SVPLREGYGRIPRGALLSMD
ALDLTDKLVSFYLETYGAEL
TANVLRDMGLQEMAGQLQAA
THQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 669 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PYRIN domain 1 | 1 |
Entities:
Entity 1, PYRIN domain 1 103 residues - 10076 Da.
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | MET | GLY | ARG | ALA | ARG | ASP | ALA | ILE | ||||
| 3 | LEU | ASP | ALA | LEU | GLU | ASN | LEU | THR | ALA | GLU | ||||
| 4 | GLU | LEU | LYS | LYS | PHE | LYS | LEU | LYS | LEU | LEU | ||||
| 5 | SER | VAL | PRO | LEU | ARG | GLU | GLY | TYR | GLY | ARG | ||||
| 6 | ILE | PRO | ARG | GLY | ALA | LEU | LEU | SER | MET | ASP | ||||
| 7 | ALA | LEU | ASP | LEU | THR | ASP | LYS | LEU | VAL | SER | ||||
| 8 | PHE | TYR | LEU | GLU | THR | TYR | GLY | ALA | GLU | LEU | ||||
| 9 | THR | ALA | ASN | VAL | LEU | ARG | ASP | MET | GLY | LEU | ||||
| 10 | GLN | GLU | MET | ALA | GLY | GLN | LEU | GLN | ALA | ALA | ||||
| 11 | THR | HIS | GLN |
Samples:
sample_1: PYRIN domain 1.0 mM; NaCl 0.05 M
Ex-cond_1: pH: 3.7; temperature: 301 K; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| w1-decoupled NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
XEASY v3.0 - peak assignment
OPAL v2.6 - energy minimization
DYANA v2.8 - structure calculation
NMR spectrometers:
- Varian Unity 800 MHz