BMRB Entry 5778

Title:
1H, 15N and 13C chemical shift assignment of the Pleckstrin Homology domain of the Protein kinase B (PKB/Akt)   PubMed: 12975590
Deposition date:
2003-04-18
Original release date:
2004-04-07
Authors:
Auguin, Daniel; Barthe, Philippe; Auge-Senegas, Marie-Therese; Hoh, Francois; Noguchi, Masayuki; Roumestand, Christian
Citation:

Citation: Auguin, Daniel; Barthe, Philippe; Auge-Senegas, Marie-Therese; Hoh, Francois; Noguchi, Masayuki; Roumestand, Christian. "Letter to the Editor: 1H, 15N and 13C Chemical Shift Assignment of the Pleckstrin Homology Domain of the Human Protein Kinase B (PKB/Akt) "  J. Biomol. NMR 27, 287-288 (2003).

Assembly members:

Assembly members:
Pleckstrin Homology domain of the human Protein Kinase B beta, polymer, 111 residues, 13214 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pleckstrin Homology domain of the human Protein Kinase B beta: MNEVSVIKEGWLHKRGEYIK TWRPRYFLLKSDGSFIGYKE RPEAPDQTLPPLNNFSVAEC QLMKTERPRPNTFVIRCLQW TTVIERTFHVDSPDEREEWM RAIQMVANSLK

Data sets:
Data typeCount
1H chemical shifts650
15N chemical shifts86
13C chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PKB-beta-PH monomer1

Entities:

Entity 1, PKB-beta-PH monomer 111 residues - 13214 Da.

1   METASNGLUVALSERVALILELYSGLUGLY
2   TRPLEUHISLYSARGGLYGLUTYRILELYS
3   THRTRPARGPROARGTYRPHELEULEULYS
4   SERASPGLYSERPHEILEGLYTYRLYSGLU
5   ARGPROGLUALAPROASPGLNTHRLEUPRO
6   PROLEUASNASNPHESERVALALAGLUCYS
7   GLNLEUMETLYSTHRGLUARGPROARGPRO
8   ASNTHRPHEVALILEARGCYSLEUGLNTRP
9   THRTHRVALILEGLUARGTHRPHEHISVAL
10   ASPSERPROASPGLUARGGLUGLUTRPMET
11   ARGALAILEGLNMETVALALAASNSERLEU
12   LYS

Samples:

sample_1: Pleckstrin Homology domain of the human Protein Kinase B beta, [U-95% 13C; U-90% 15N], 0.3 – 0.4 mM

cond_set_1: pH: 7.4; temperature: 286 K; ionic strength: 0.3 M

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablecond_set_1
HN(CO)CAsample_1not availablecond_set_1
HNCACBsample_1not availablecond_set_1
CBCA(CO)NHsample_1not availablecond_set_1
HNCOsample_1not availablecond_set_1
HN(CA)COsample_1not availablecond_set_1
H(CC)H-TOCSYsample_1not availablecond_set_1
1H-15N NOESYsample_1not availablecond_set_1
1H-13C NOESYsample_1not availablecond_set_1

Software:

GIFA v4.4 - NMR processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA06280 BAE25779 BAE34668 BAG37185 BAH14135
GB AAA36585 AAA58364 AAA83557 AAH26151 AAH32709
REF NP_001103678 NP_001167450 NP_001193075 NP_001243708 NP_001252918
SP P31751 P47197 Q60823
TPG DAA19835
AlphaFold Q60823

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts