BMRB Entry 5766

Name: Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda
Published: 2003-09-08

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PDB ID: 1nzp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5766
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda

Deposition date:

2003-04-11

Original release date:

2003-09-08

Authors:

DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.

Citation:

Citation: DeRose, E.; Kirby, T.; Mueller, G.; Bebenek, K.; Garcia-Diaz, M.; Blanco, L.; Kunkel, T.; London, R.. "Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda" Biochemistry 42, 9564-9574 (2003).
PubMed: 12911298

Assembly members:

Assembly members:
50S Ribosomal Protein L18, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET30A(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
50S Ribosomal Protein L18: MAQPSSQKATNHNLHITEKL EVLAKAYSVQGDKWRALGYA KAINALKSFHKPVTSYQEAC SIPGIGKRMAEKIIEILESG HLRKLDH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	440
13C chemical shifts	328
15N chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	50S Ribosomal Protein L18	1

Entities:

Entity 1, 50S Ribosomal Protein L18 87 residues - Formula weight is not available

1

MET

ALA

GLN

PRO

SER

GLN

LYS

ALA

THR

2

ASN

HIS

ASN

LEU

HIS

ILE

THR

GLU

LYS

LEU

3

GLU

VAL

LEU

ALA

LYS

ALA

TYR

SER

VAL

GLN

4

GLY

ASP

LYS

TRP

ARG

ALA

LEU

GLY

TYR

ALA

5

LYS

ALA

ILE

ASN

ALA

LEU

LYS

SER

PHE

HIS

6

LYS

PRO

VAL

THR

SER

TYR

GLN

GLU

ALA

CYS

7

SER

ILE

PRO

GLY

ILE

GLY

LYS

ARG

MET

ALA

8

GLU

LYS

ILE

GLU

ILE

LEU

GLU

SER

GLY

9

HIS

LEU

ARG

LYS

LEU

ASP

HIS

Samples:

sample_1: 50S Ribosomal Protein L18, [U-15N; U-13C], 2 mM; Tris-d11 5 mM; NaCl 100 mM; DTT 1 mM; NaN3 5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 110 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available

Software:

VNMR v6.1C - collection

NMRPipe v2.1 - processing

NMRView v5.0.4 - data analysis

CNS v1.0 - structure solution

ARIA v1.1 - structure solution, refinement

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1NZP 1RZT 1XSL 1XSN 1XSP 2BCQ 2BCR 2BCS 2BCU 2BCV 2GWS 2PFN 2PFO 2PFP 2PFQ 3C5F 3C5G 3HW8 3HWT 3HX0 3MDA 3MDC 3MGH 3MGI 3PML 3PMN 3PNC 3UPQ 3UQ0 3UQ2 4FO6 4K4G 4K4H 4K4I
DBJ	BAB13852 BAB14050 BAE02437 BAG10573 BAK63678
EMBL	CAB65074
GB	AAF27541 AAG22519 AAH68529 AAM77696 ADQ01128
REF	NP_001167555 NP_001253835 NP_001267179 NP_001270218 NP_037406
SP	Q4R380 Q9UGP5
AlphaFold	Q4R380 Q9UGP5