BMRB Entry 5764

Name: 1H, 13C and 15N Chemical Shift Assignments for the Nuclear Factor ALY RBD Domain
Published: 2003-08-08

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PDB ID: 1no8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5764
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignments for the Nuclear Factor ALY RBD Domain

Deposition date:

2003-04-07

Original release date:

2003-08-08

Authors:

Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter

Citation:

Citation: Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter. "Structure of the Nuclear Factor ALY: Insights into Post-transcriptional Regulatory and mRNA Nuclear Export Processes" Biochemistry 42, 7348-7357 (2003).
PubMed: 12809490

Assembly members:

Assembly members:
ALY, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia Escherichia coli Vector: pet21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ALY: SRPKQLPDKWQHDLFDSGFG GGAGVETGGKLLVSNLDFGV SDADIQELFAEFGTLKKAAV HYDRSGRSLGTADVHFERKA DALKAMKQYNGVPLDGRPMN IQLVTS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	699
13C chemical shifts	419
15N chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ALY monomer	1

Entities:

Entity 1, ALY monomer 106 residues - Formula weight is not available

1

SER

ARG

PRO

LYS

GLN

LEU

PRO

ASP

LYS

TRP

2

GLN

HIS

ASP

LEU

PHE

ASP

SER

GLY

PHE

GLY

3

GLY

ALA

GLY

VAL

GLU

THR

GLY

LYS

4

LEU

VAL

SER

ASN

LEU

ASP

PHE

GLY

VAL

5

SER

ASP

ALA

ASP

ILE

GLN

GLU

LEU

PHE

ALA

6

GLU

PHE

GLY

THR

LEU

LYS

ALA

VAL

7

HIS

TYR

ASP

ARG

SER

GLY

ARG

SER

LEU

GLY

8

THR

ALA

ASP

VAL

HIS

PHE

GLU

ARG

LYS

ALA

9

ASP

ALA

LEU

LYS

ALA

MET

LYS

GLN

TYR

ASN

10

GLY

VAL

PRO

LEU

ASP

GLY

ARG

PRO

MET

ASN

11

ILE

GLN

LEU

VAL

THR

SER

Samples:

sample_1: ALY, [U-15N], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: ALY, [U-15N; U-13C], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_2: pH: 6.5; temperature: 289 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_3: pH: 5.9; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated_NOESY	not available	not available	not available
3D 13C-HMQC-NOESY	not available	not available	not available
3D 15N/13C-NOESY-HSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
C(CO)NH-TOCSY	not available	not available	not available
HC(CO)NH-TOCSY	not available	not available	not available
HNHA	not available	not available	not available
HCCH-COSY	not available	not available	not available
CCH-COSY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HNHB	not available	not available	not available
2D TOCSY	not available	not available	not available
3D 13C-separated_NOESY	not available	not available	not available

Software:

XWINNMR v2.1 - collection

NMRPipe v1.8, 2.1 - processing

NMRDraw v1.8, 2.1 - processing

NMRVIEW v3 - data analysis

DYANA v1.5 - structure solution

AMBER v6, 7 - refinement

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz
Bruker AMX 500 MHz

PDB	1NO8 3ULH
EMBL	CAB76383 CAJ81934
GB	AAC53117 AAD09608 AAH52302 AAH92013 AAI02385
REF	NP_001017027 NP_001030494 NP_001089314 NP_001103072 NP_001232343
SP	B5FXN8 O08583 Q28FB9 Q3T0I4 Q58EA2
TPG	DAA18257
AlphaFold	B5FXN8 O08583 Q28FB9 Q3T0I4 Q58EA2