BMRB Entry 5762

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5762

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Title:
The alternatively spliced PDZ2 domain of PTP-BL, PDZ2as
Deposition date:
2003-04-07
Original release date:
2011-08-11
Authors:
Walma, Tine; Aelen, Jan; Oostendorp, M.; Vuister, Geerten
Citation:

Citation: Walma, Tine; Aelen, Jan; Nabuurs, S.; Oostendorp, Marlies; van den Berk, L.; Hendriks, W.; Vuister, Geerten. "A Closed Binding Pocket and Global Destabilization Modify the Binding Properties of an Alternatively Spliced Form of the Second PDZ Domain of PTP-BL"  Structure (Cambridge, MA, U. S.) 12, 11-20 (2004).
PubMed: 14725761

Assembly members:

Assembly members:
Second PDZ Domain from PTP-BL, polymer, 99 residues, 10355 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Second PDZ Domain from PTP-BL: KPGDTFEVELAKTDGSLGIS VTVLFDKGGVNTSVRHGGIY VKAIIPKGAAESDGRIHKGD RVLAVNGVSLEGATHKQAVE TLRNTGQVVHLLLEKGQVP

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts108
1H chemical shifts671
coupling constants58
heteronuclear NOE values90
H exchange protection factors55
residual dipolar couplings66
T1 relaxation values88
T2 relaxation values86

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ2-AS1

Entities:

Entity 1, PDZ2-AS 99 residues - 10355 Da.

1   LYSPROGLYASPTHRPHEGLUVALGLULEU
2   ALALYSTHRASPGLYSERLEUGLYILESER
3   VALTHRVALLEUPHEASPLYSGLYGLYVAL
4   ASNTHRSERVALARGHISGLYGLYILETYR
5   VALLYSALAILEILEPROLYSGLYALAALA
6   GLUSERASPGLYARGILEHISLYSGLYASP
7   ARGVALLEUALAVALASNGLYVALSERLEU
8   GLUGLYALATHRHISLYSGLNALAVALGLU
9   THRLEUARGASNTHRGLYGLNVALVALHIS
10   LEULEULEUGLULYSGLYGLNVALPRO

Related Database Links:

PDB 1OZI

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks