BMRB Entry 5762

Name: The alternatively spliced PDZ2 domain of PTP-BL, PDZ2as
Published: 2011-08-11

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5762

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The alternatively spliced PDZ2 domain of PTP-BL, PDZ2as

Deposition date:

2003-04-07

Original release date:

2011-08-11

Authors:

Walma, Tine; Aelen, Jan; Oostendorp, M.; Vuister, Geerten

Citation:

Citation: Walma, Tine; Aelen, Jan; Nabuurs, S.; Oostendorp, Marlies; van den Berk, L.; Hendriks, W.; Vuister, Geerten. "A Closed Binding Pocket and Global Destabilization Modify the Binding Properties of an Alternatively Spliced Form of the Second PDZ Domain of PTP-BL" Structure (Cambridge, MA, U. S.) 12, 11-20 (2004).
PubMed: 14725761

Assembly members:

Assembly members:
Second PDZ Domain from PTP-BL, polymer, 99 residues, 10355 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Second PDZ Domain from PTP-BL: KPGDTFEVELAKTDGSLGIS VTVLFDKGGVNTSVRHGGIY VKAIIPKGAAESDGRIHKGD RVLAVNGVSLEGATHKQAVE TLRNTGQVVHLLLEKGQVP

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_coupling_constants_1
heteronucl_NOEs
- heteronuclear_NOE
heteronucl_T1_relaxation
- T1_relaxation_label
heteronucl_T2_relaxation
- T2_relaxation_label
H_exch_protection_factors
- H_exchange_protection_factor_2
H_exch_rates
RDCs
- residual_dipolar_couplings

Data type	Count
13C chemical shifts	297
15N chemical shifts	108
1H chemical shifts	671
coupling constants	58
heteronuclear NOE values	90
H exchange protection factors	55
residual dipolar couplings	66
T1 relaxation values	88
T2 relaxation values	86

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ2-AS	1

Entities:

Entity 1, PDZ2-AS 99 residues - 10355 Da.

1

LYS

PRO

GLY

ASP

THR

PHE

GLU

VAL

GLU

LEU

2

ALA

LYS

THR

ASP

GLY

SER

LEU

GLY

ILE

SER

3

VAL

THR

VAL

LEU

PHE

ASP

LYS

GLY

VAL

4

ASN

THR

SER

VAL

ARG

HIS

GLY

ILE

TYR

5

VAL

LYS

ALA

ILE

PRO

LYS

GLY

ALA

6

GLU

SER

ASP

GLY

ARG

ILE

HIS

LYS

GLY

ASP

7

ARG

VAL

LEU

ALA

VAL

ASN

GLY

VAL

SER

LEU

8

GLU

GLY

ALA

THR

HIS

LYS

GLN

ALA

VAL

GLU

9

THR

LEU

ARG

ASN

THR

GLY

GLN

VAL

HIS

10

LEU

GLU

LYS

GLY

GLN

VAL

PRO

Samples:

sample_1: Second PDZ Domain from PTP-BL, [U-2H; U-15N; U-13C], 1.4 mM; K2HPO4/KH2PO4 50 mM; KCl 50 mM

sample_2: Second PDZ Domain from PTP-BL, [U-2H; U-15N], 1.1 mM; CH3COO- 50 mM; KCl 50 mM

condition_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

condition_2: ionic strength: 0.1 M; pH: 3.5; pressure: 1 atm; temperature: 281.5 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Varian Inova 800 MHz