BMRB Entry 5761

Name: 1H, 13C and 15N Chemical Shift Assignments for Gamma-Adaptin Ear Domain
Published: 2003-12-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5761

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignments for Gamma-Adaptin Ear Domain

Deposition date:

2003-04-07

Original release date:

2003-12-19

Authors:

Denisov, Alexei; Gehring, Kalle

Citation:

Citation: Wasiak, Sylwia; Denisov, Alexei; Han, Z.; Leventis, P.; Heuvel, E.; Boulianne, G.; Kay, B.; Gehring, Kalle; McPherson, Peter. "Characterization of a gamma-adaptin ear-binding motif in enthoprotin" FEBS Lett. 555, 437-442 (2003).

Assembly members:

Assembly members:
gamma-adaptin ear domain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGET-2TK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gamma-adaptin ear domain: IPSITAYSKNGLKIEFTFER SNTNPSVTVITIQASNSTEL DMTDFVFQAAVPKTFQLQLL SPSSSVVPAFNTGTITQVIK VLNPQKQQLRMRIKLTYNHK GSAMQDLAEVNNFPPQSWQ

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	393
13C chemical shifts	330
15N chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gamma-ear monomer	1

Entities:

Entity 1, gamma-ear monomer 119 residues - Formula weight is not available

1

ILE

PRO

SER

ILE

THR

ALA

TYR

SER

LYS

ASN

2

GLY

LEU

LYS

ILE

GLU

PHE

THR

PHE

GLU

ARG

3

SER

ASN

THR

ASN

PRO

SER

VAL

THR

VAL

ILE

4

THR

ILE

GLN

ALA

SER

ASN

SER

THR

GLU

LEU

5

ASP

MET

THR

ASP

PHE

VAL

PHE

GLN

ALA

6

VAL

PRO

LYS

THR

PHE

GLN

LEU

GLN

LEU

7

SER

PRO

SER

VAL

PRO

ALA

PHE

8

ASN

THR

GLY

THR

ILE

THR

GLN

VAL

ILE

LYS

9

VAL

LEU

ASN

PRO

GLN

LYS

GLN

LEU

ARG

10

MET

ARG

ILE

LYS

LEU

THR

TYR

ASN

HIS

LYS

11

GLY

SER

ALA

MET

GLN

ASP

LEU

ALA

GLU

VAL

12

ASN

PHE

PRO

GLN

SER

TRP

GLN

Samples:

sample_1: gamma-adaptin ear domain, [U-99% 13C; U-99% 15N], 1.5 mM; sodium phosphate buffer 50 mM; NaCl 150 mM

sample_2: gamma-adaptin ear domain, [U-99% 15N], 1.5 mM; sodium phosphate buffer 50 mM; NaCl 150 mM

Ex-cond_1: pH: 7.2; temperature: 303 K; ionic strength: 0.20 M

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	not available	not available	not available
3D CBCA(CO)HN	not available	not available	not available
3D HNCO	not available	not available	not available
3D 15N-HMQC-NOESY	not available	not available	not available
3D 15N-HMQC-TOCSY	not available	not available	not available
2D 15N-HSQC	not available	not available	not available

Software:

XWINNMR v3.1 - collection, processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1GYU 1GYV 1GYW 1IU1 2A7B
DBJ	BAA33389 BAB86336 BAC25904 BAC28629 BAC29406
EMBL	CAA38296 CAA72902 CAB53673 CAH92944
GB	AAH36283 AAH52703 AAH54535 AAI66845 ACE86968
PRF	1704251A
REF	NP_001025178 NP_001119 NP_001126734 NP_001178365 NP_001288140
SP	O43747 P22892 Q5R5M2
TPG	DAA20238 DAA20239
AlphaFold	O43747 P22892 Q5R5M2