BMRB Entry 5761

Title:
1H, 13C and 15N Chemical Shift Assignments for Gamma-Adaptin Ear Domain
Deposition date:
2003-04-07
Original release date:
2003-12-19
Authors:
Denisov, Alexei; Gehring, Kalle
Citation:

Citation: Wasiak, Sylwia; Denisov, Alexei; Han, Z.; Leventis, P.; Heuvel, E.; Boulianne, G.; Kay, B.; Gehring, Kalle; McPherson, Peter. "Characterization of a gamma-adaptin ear-binding motif in enthoprotin"  FEBS Lett. 555, 437-442 (2003).

Assembly members:

Assembly members:
gamma-adaptin ear domain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGET-2TK

Data sets:
Data typeCount
1H chemical shifts393
13C chemical shifts330
15N chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gamma-ear monomer1

Entities:

Entity 1, gamma-ear monomer 119 residues - Formula weight is not available

1   ILEPROSERILETHRALATYRSERLYSASN
2   GLYLEULYSILEGLUPHETHRPHEGLUARG
3   SERASNTHRASNPROSERVALTHRVALILE
4   THRILEGLNALASERASNSERTHRGLULEU
5   ASPMETTHRASPPHEVALPHEGLNALAALA
6   VALPROLYSTHRPHEGLNLEUGLNLEULEU
7   SERPROSERSERSERVALVALPROALAPHE
8   ASNTHRGLYTHRILETHRGLNVALILELYS
9   VALLEUASNPROGLNLYSGLNGLNLEUARG
10   METARGILELYSLEUTHRTYRASNHISLYS
11   GLYSERALAMETGLNASPLEUALAGLUVAL
12   ASNASNPHEPROPROGLNSERTRPGLN

Samples:

sample_1: gamma-adaptin ear domain, [U-99% 13C; U-99% 15N], 1.5 mM; sodium phosphate buffer 50 mM; NaCl 150 mM

sample_2: gamma-adaptin ear domain, [U-99% 15N], 1.5 mM; sodium phosphate buffer 50 mM; NaCl 150 mM

Ex-cond_1: pH: 7.2; temperature: 303 K; ionic strength: 0.20 M

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)HNnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D 15N-HMQC-NOESYnot availablenot availablenot available
3D 15N-HMQC-TOCSYnot availablenot availablenot available
2D 15N-HSQCnot availablenot availablenot available

Software:

XWINNMR v3.1 - collection, processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA33389 BAB86336 BAC25904 BAC28629 BAC29406
EMBL CAA38296 CAA72902 CAB53673 CAH92944
GB AAH36283 AAH52703 AAH54535 AAI66845 ACE86968
PRF 1704251A
REF NP_001025178 NP_001119 NP_001126734 NP_001178365 NP_001288140
SP O43747 P22892 Q5R5M2
TPG DAA20238 DAA20239
AlphaFold O43747 P22892 Q5R5M2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks