BMRB Entry 5755

Title:
Assignment of Human Phosphatase Regenerating Liver 2 (PRL-2)
Deposition date:
2003-03-24
Original release date:
2003-09-28
Authors:
Zhou, Hongjun; Gallina, Marijo; Mao, Helen; Betz, Stephen; Fetrow, Jacquelyn; Domaille, Peter
Citation:

Citation: Zhou, Hongjun; Gallina, Marijo; Mao, Hongyuan; Nietlispach, Daniel; Betz, Stephen; Fetrow, Jacquelyn; Domaille, Peter. "Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary structure of the human protein tyrosine phosphatase, PRL-2"  J. Biomol. NMR 27, 397-398 (2003).

Assembly members:

Assembly members:
Phosphatse Regenerating Liver 2, polymer, 172 residues, 22310 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1027
13C chemical shifts672
15N chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PRL-21

Entities:

Entity 1, PRL-2 172 residues - 22310 Da.

1   GLYSERALASERASNARGPROALAPROVAL
2   GLUILESERTYRGLUASNMETARGPHELEU
3   ILETHRHISASNPROTHRASNALATHRLEU
4   ASNLYSPHETHRGLUGLULEULYSLYSTYR
5   GLYVALTHRTHRLEUVALARGVALCYSASP
6   ALATHRTYRASPLYSALAPROVALGLULYS
7   GLUGLYILEHISVALLEUASPTRPPROPHE
8   ASPASPGLYALAPROPROPROASNGLNILE
9   VALASPASPTRPLEUASNLEULEULYSTHR
10   LYSPHEARGGLUGLUPROGLYCYSCYSVAL
11   ALAVALHISCYSVALALAGLYLEUGLYARG
12   ALAPROVALLEUVALALALEUALALEUILE
13   GLUCYSGLYMETLYSTYRGLUASPALAVAL
14   GLNPHEILEARGGLNLYSARGARGGLYALA
15   PHEASNSERLYSGLNLEULEUTYRLEUGLU
16   LYSTYRARGPROLYSMETARGLEUARGPHE
17   ARGASPTHRASNGLYHISCYSCYSVALGLN
18   LEUGLU

Samples:

sample_1: Phosphatse Regenerating Liver 2, [U-13C; U-15N], 0.6 – 1.4 mM; sodium phosphate 50 mM; DTT-d10 10 mM; EDTA-d16 1 mM; sodium azide 0.01%

condition_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCNH NOESYnot availablenot availablenot available

Software:

nmrPipe - data processing

Azara v2.6 - data processing

Ansig4OpenGL v2.6 - data processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAC28149 BAC32217 BAE22604 BAE33489 BAE88131
EMBL CAA07417 CAL37487 CAL38409 CAL38433
GB AAA90979 AAB39331 AAB40598 AAB42170 AAB59575
REF NP_001098468 NP_001158217 NP_001231270 NP_001272163 NP_033000
SP O70274 Q12974 Q6P9X4
TPG DAA32316
AlphaFold O70274 Q12974 Q6P9X4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks