BMRB Entry 5755
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5755
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zhou, Hongjun; Gallina, Marijo; Mao, Hongyuan; Nietlispach, Daniel; Betz, Stephen; Fetrow, Jacquelyn; Domaille, Peter. "Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary
structure of the human protein tyrosine phosphatase, PRL-2" J. Biomol. NMR 27, 397-398 (2003).
Assembly members:
Phosphatse Regenerating Liver 2, polymer, 172 residues, 22310 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Phosphatse Regenerating Liver 2: GSASNRPAPVEISYENMRFL
ITHNPTNATLNKFTEELKKY
GVTTLVRVCDATYDKAPVEK
EGIHVLDWPFDDGAPPPNQI
VDDWLNLLKTKFREEPGCCV
AVHCVAGLGRAPVLVALALI
ECGMKYEDAVQFIRQKRRGA
FNSKQLLYLEKYRPKMRLRF
RDTNGHCCVQLE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1027 |
| 13C chemical shifts | 672 |
| 15N chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PRL-2 | 1 |
Entities:
Entity 1, PRL-2 172 residues - 22310 Da.
| 1 | GLY | SER | ALA | SER | ASN | ARG | PRO | ALA | PRO | VAL | ||||
| 2 | GLU | ILE | SER | TYR | GLU | ASN | MET | ARG | PHE | LEU | ||||
| 3 | ILE | THR | HIS | ASN | PRO | THR | ASN | ALA | THR | LEU | ||||
| 4 | ASN | LYS | PHE | THR | GLU | GLU | LEU | LYS | LYS | TYR | ||||
| 5 | GLY | VAL | THR | THR | LEU | VAL | ARG | VAL | CYS | ASP | ||||
| 6 | ALA | THR | TYR | ASP | LYS | ALA | PRO | VAL | GLU | LYS | ||||
| 7 | GLU | GLY | ILE | HIS | VAL | LEU | ASP | TRP | PRO | PHE | ||||
| 8 | ASP | ASP | GLY | ALA | PRO | PRO | PRO | ASN | GLN | ILE | ||||
| 9 | VAL | ASP | ASP | TRP | LEU | ASN | LEU | LEU | LYS | THR | ||||
| 10 | LYS | PHE | ARG | GLU | GLU | PRO | GLY | CYS | CYS | VAL | ||||
| 11 | ALA | VAL | HIS | CYS | VAL | ALA | GLY | LEU | GLY | ARG | ||||
| 12 | ALA | PRO | VAL | LEU | VAL | ALA | LEU | ALA | LEU | ILE | ||||
| 13 | GLU | CYS | GLY | MET | LYS | TYR | GLU | ASP | ALA | VAL | ||||
| 14 | GLN | PHE | ILE | ARG | GLN | LYS | ARG | ARG | GLY | ALA | ||||
| 15 | PHE | ASN | SER | LYS | GLN | LEU | LEU | TYR | LEU | GLU | ||||
| 16 | LYS | TYR | ARG | PRO | LYS | MET | ARG | LEU | ARG | PHE | ||||
| 17 | ARG | ASP | THR | ASN | GLY | HIS | CYS | CYS | VAL | GLN | ||||
| 18 | LEU | GLU |
Samples:
sample_1: Phosphatse Regenerating Liver 2, [U-13C; U-15N], 0.6 – 1.4 mM; sodium phosphate 50 mM; DTT-d10 10 mM; EDTA-d16 1 mM; sodium azide 0.01%
condition_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HCC(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCNH NOESY | not available | not available | not available |
Software:
nmrPipe - data processing
Azara v2.6 - data processing
Ansig4OpenGL v2.6 - data processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| DBJ | BAC28149 BAC32217 BAE22604 BAE33489 BAE88131 |
| EMBL | CAA07417 CAL37487 CAL38409 CAL38433 |
| GB | AAA90979 AAB39331 AAB40598 AAB42170 AAB59575 |
| REF | NP_001098468 NP_001158217 NP_001231270 NP_001272163 NP_033000 |
| SP | O70274 Q12974 Q6P9X4 |
| TPG | DAA32316 |
| AlphaFold | O70274 Q12974 Q6P9X4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks