BMRB Entry 5754

Name: 1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain
Published: 2004-04-07

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5754

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain

Deposition date:

2003-03-24

Original release date:

2004-04-07

Authors:

Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald

Citation:

Citation: Vogtherr, Martin; Grimme, Susanne; Pescatore, Barbara; Schwalbe, Harald. "Letter to the Editor: 1H, 13C and 15N Backbone Resonance Assignment of the VASP EVH1 Domain" J. Biomol. NMR 27, 189-190 (2003).
PubMed: 12913419

Assembly members:

Assembly members:
Human VASP Ena-Vasp homology domain 1, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human VASP Ena-Vasp homology domain 1: MSETVICSSRATVMLYDDGN KRWLPAGTGPQAFSRVQIYH NPTANSFRVVGRKMQPDQQV VINCAIVRGVKYNQATPNFH QWRDARQVWGLNFGSKEDAA QFAAGMASALEALEG

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
15N chemical shifts	110
1H chemical shifts	460
13C chemical shifts	332

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VASP EVH1 domain	1

Entities:

Entity 1, VASP EVH1 domain 115 residues - Formula weight is not available

1

MET

SER

GLU

THR

VAL

ILE

CYS

SER

ARG

2

ALA

THR

VAL

MET

LEU

TYR

ASP

GLY

ASN

3

LYS

ARG

TRP

LEU

PRO

ALA

GLY

THR

GLY

PRO

4

GLN

ALA

PHE

SER

ARG

VAL

GLN

ILE

TYR

HIS

5

ASN

PRO

THR

ALA

ASN

SER

PHE

ARG

VAL

6

GLY

ARG

LYS

MET

GLN

PRO

ASP

GLN

VAL

7

VAL

ILE

ASN

CYS

ALA

ILE

VAL

ARG

GLY

VAL

8

LYS

TYR

ASN

GLN

ALA

THR

PRO

ASN

PHE

HIS

9

GLN

TRP

ARG

ASP

ALA

ARG

GLN

VAL

TRP

GLY

10

LEU

ASN

PHE

GLY

SER

LYS

GLU

ASP

ALA

11

GLN

PHE

ALA

GLY

MET

ALA

SER

ALA

LEU

12

GLU

ALA

LEU

GLU

GLY

Samples:

sample_1: Human VASP Ena-Vasp homology domain 1, [U-13C; U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM

sample_2: Human VASP Ena-Vasp homology domain 1, [U-15N], 1.0 mM; DTT 10 mM; KCl 50 mM; KPi 20 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCO	not available	not available	Ex-cond_1
HN(CA)CO	not available	not available	Ex-cond_1
CBCA(CO)NH	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
HCC(CO)NH-TOCSY	not available	not available	Ex-cond_1

Software:

xwinnmr v2.9 - acqusition, processing

xeasy v1.3.14 - peak assignment

talos v1999.019.15.47 - assignment, structure validation

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	18569
PDB	1EGX
DBJ	BAG37336 BAJ21098
EMBL	CAA67147 CAA86523
GB	AAH26019 AAH38224 AIC59345 EAW57362 EAW57363
REF	NP_003361 XP_001106536 XP_002829453 XP_003277690 XP_003817563
SP	P50552
AlphaFold	P50552