BMRB Entry 5743

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for the extracellular domain of b-dystroglycan (654-750)
Deposition date:
2003-03-17
Original release date:
2003-12-18
Authors:
Bozzi, Manuela; Brancaccio, Andrea; Paci, Maurizio; Cicero, Daniel
Citation:

Citation: Bozzi, Manuela; Bianchi, Marzia; Sciandra, Francesca; Paci, Maurizio; Giardina, Bruno; Brancaccio, Andrea; Cicero, Daniel. "Structural Characterization by NMR of the Natively Unfolded Extracellular Domain of beta-Dystroglycan: Toward the Identification of the Binding Epitope for alpha-Dystroglycan"  Biochemistry 42, 13717-13724 (2003).
PubMed: 14622018

Assembly members:

Assembly members:
extracellular domain of beta subunit of dystroglycan, polymer, 99 residues, 10528.72 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
extracellular domain of beta subunit of dystroglycan: GSSIVVEWTNNTLPLEPCPK EQIIGLSRRIADENGKPRPA FSNALEPDFKALSIAVTGSG SCRHLQFIPVAPPSPGSSAA PATEVPDRDPEKSSEDDVY

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts76
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1extracellular domain of beta subunit of dystroglycan, b-DG(654-750)1

Entities:

Entity 1, extracellular domain of beta subunit of dystroglycan, b-DG(654-750) 99 residues - 10528.72 Da.

1   GLYSERSERILEVALVALGLUTRPTHRASN
2   ASNTHRLEUPROLEUGLUPROCYSPROLYS
3   GLUGLNILEILEGLYLEUSERARGARGILE
4   ALAASPGLUASNGLYLYSPROARGPROALA
5   PHESERASNALALEUGLUPROASPPHELYS
6   ALALEUSERILEALAVALTHRGLYSERGLY
7   SERCYSARGHISLEUGLNPHEILEPROVAL
8   ALAPROPROSERPROGLYSERSERALAALA
9   PROALATHRGLUVALPROASPARGASPPRO
10   GLULYSSERSERGLUASPASPVALTYR

Samples:

sample_1: extracellular domain of beta subunit of dystroglycan, [U-13C; U-15N], 0.3 – 0.5 mM; sodium phosphate 20 mM; sodium chloride 0.15 M

Ex_conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-TOCSY-HSQCsample_1not availableEx_conditions_1
3D HNNsample_1not availableEx_conditions_1
3D HNCOsample_1not availableEx_conditions_1
3D HNCAsample_1not availableEx_conditions_1
3D HN(CO)CAsample_1not availableEx_conditions_1
3D CBCANHsample_1not availableEx_conditions_1
3D CBCA(CO)NHsample_1not availableEx_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 400 MHz
  • Bruker Avance 700 MHz

Related Database Links:

DBJ BAC29332 BAC30105 BAC35576
GB AAA99779 AAC52853 AAH07150 EDL21272 EGW08936
REF NP_001263410 NP_001263411 NP_001263414 NP_001263415 NP_001263421
SP Q62165
AlphaFold Q62165

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks