BMRB Entry 5743

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5743

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Chemical Shift Assignments for the extracellular domain of b-dystroglycan (654-750)

Deposition date:

2003-03-17

Original release date:

2003-12-18

Authors:

Bozzi, Manuela; Brancaccio, Andrea; Paci, Maurizio; Cicero, Daniel

Citation:

Citation: Bozzi, Manuela; Bianchi, Marzia; Sciandra, Francesca; Paci, Maurizio; Giardina, Bruno; Brancaccio, Andrea; Cicero, Daniel. "Structural Characterization by NMR of the Natively Unfolded Extracellular Domain of beta-Dystroglycan: Toward the Identification of the Binding Epitope for alpha-Dystroglycan" Biochemistry 42, 13717-13724 (2003).
PubMed: 14622018

Assembly members:

Assembly members:
extracellular domain of beta subunit of dystroglycan, polymer, 99 residues, 10528.72 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
extracellular domain of beta subunit of dystroglycan: GSSIVVEWTNNTLPLEPCPK EQIIGLSRRIADENGKPRPA FSNALEPDFKALSIAVTGSG SCRHLQFIPVAPPSPGSSAA PATEVPDRDPEKSSEDDVY

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	76
1H chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	extracellular domain of beta subunit of dystroglycan, b-DG(654-750)	1

Entities:

Entity 1, extracellular domain of beta subunit of dystroglycan, b-DG(654-750) 99 residues - 10528.72 Da.

1

GLY

SER

ILE

VAL

GLU

TRP

THR

ASN

2

ASN

THR

LEU

PRO

LEU

GLU

PRO

CYS

PRO

LYS

3

GLU

GLN

ILE

GLY

LEU

SER

ARG

ILE

4

ALA

ASP

GLU

ASN

GLY

LYS

PRO

ARG

PRO

ALA

5

PHE

SER

ASN

ALA

LEU

GLU

PRO

ASP

PHE

LYS

6

ALA

LEU

SER

ILE

ALA

VAL

THR

GLY

SER

GLY

7

SER

CYS

ARG

HIS

LEU

GLN

PHE

ILE

PRO

VAL

8

ALA

PRO

SER

PRO

GLY

SER

ALA

9

PRO

ALA

THR

GLU

VAL

PRO

ASP

ARG

ASP

PRO

10

GLU

LYS

SER

GLU

ASP

VAL

TYR

Samples:

sample_1: extracellular domain of beta subunit of dystroglycan, [U-13C; U-15N], 0.3 – 0.5 mM; sodium phosphate 20 mM; sodium chloride 0.15 M

Ex_conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-TOCSY-HSQC	sample_1	not available	Ex_conditions_1
3D HNN	sample_1	not available	Ex_conditions_1
3D HNCO	sample_1	not available	Ex_conditions_1
3D HNCA	sample_1	not available	Ex_conditions_1
3D HN(CO)CA	sample_1	not available	Ex_conditions_1
3D CBCANH	sample_1	not available	Ex_conditions_1
3D CBCA(CO)NH	sample_1	not available	Ex_conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 400 MHz
Bruker Avance 700 MHz

DBJ	BAC29332 BAC30105 BAC35576
GB	AAA99779 AAC52853 AAH07150 EDL21272 EGW08936
REF	NP_001263410 NP_001263411 NP_001263414 NP_001263415 NP_001263421
SP	Q62165
AlphaFold	Q62165