BMRB Entry 5722

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PDB ID: 1oqp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5722
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignmens of Ca2+/C-terminal Domain of Caltractin (1H, 13C, and 15N) in Complex with the Cdc31p-Binding Domain from Kar1p (1H)

Deposition date:

2003-03-10

Original release date:

2003-06-25

Authors:

Hu, Haitao; Chazin, Walter

Citation:

Citation: Hu, Haitao; Chazin, Walter. "Unique Features in the C-terminal Domain Provide Caltractin with Target Specificity" J. Mol. Biol. 330, 473-484 (2003).
PubMed: 12842464

Assembly members:

Assembly members:
C-terminal domain of caltractin, polymer, 77 residues, 8966 Da.
19-residue cdc31p-binding domain from Kar1p, polymer, 19 residues, 2492 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: green algae Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: not available Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal domain of caltractin: GSGERDSREEILKAFRLFDD DNSGTITIKDLRRVAKELGE NLTEEELQEMIAEADRNDDN EIDEDEFIRIMKKTSLF
19-residue cdc31p-binding domain from Kar1p: KKRELIESKWHRLLFHDKK

Data sets:

assigned_chemical_shifts
- shift_set_1
- shift_set_2

Data type	Count
1H chemical shifts	686
13C chemical shifts	317
15N chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal domain of caltractin	1
2	19-residue Kar1p peptide	2
3	CALCIUM (II) ION	3

Entities:

Entity 1, C-terminal domain of caltractin 77 residues - 8966 Da.

1

GLY

SER

GLY

GLU

ARG

ASP

SER

ARG

GLU

2

ILE

LEU

LYS

ALA

PHE

ARG

LEU

PHE

ASP

3

ASP

ASN

SER

GLY

THR

ILE

THR

ILE

LYS

ASP

4

LEU

ARG

VAL

ALA

LYS

GLU

LEU

GLY

GLU

5

ASN

LEU

THR

GLU

LEU

GLN

GLU

MET

6

ILE

ALA

GLU

ALA

ASP

ARG

ASN

ASP

ASN

7

GLU

ILE

ASP

GLU

ASP

GLU

PHE

ILE

ARG

ILE

8

MET

LYS

THR

SER

LEU

PHE

Entity 2, 19-residue Kar1p peptide 19 residues - 2492 Da.

1

LYS

ARG

GLU

LEU

ILE

GLU

SER

LYS

TRP

2

HIS

ARG

LEU

PHE

HIS

ASP

LYS

Entity 3, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: C-terminal domain of caltractin, [U-13C; U-15N], 1.0 mM; 19-residue cdc31p-binding domain from Kar1p 1.2 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

PDB	1OQP 1OQP
DBJ	GAA25914
EMBL	CAA96082 CAY82415 CAA96082
GB	AAA34716 AAT92901 AHY76913 EDN62631 EDV12432
REF	NP_014211 NP_014211
SP	P11927
TPG	DAA10365
SWISS-PROT	P11927
GenBank	EDN62631 EDV12432 AAA34716 AAT92901
AlphaFold	P11927