BMRB Entry 5712

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5712

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment for human eIF4E in complex with m7GDP and a 17-amino acid peptide derived from human eIF4G

Deposition date:

2003-03-02

Original release date:

2006-05-30

Authors:

Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo

Citation:

Citation: Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo. "Letter to the Editor: Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII " J. Biomol. NMR 27, 279-280 (2003).

Assembly members:

Assembly members:
translation initiation factor 4E, polymer, 217 residues, 25097 Da.
eIF4E binding region, polymer, 17 residues, Formula weight is not available
M7G, non-polymer, 459.243 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
translation initiation factor 4E: MATVEPETTPTPNPPTTEEE KTESNQEVANPEHYIKHPLQ NRWALWFFKNDKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSNLMPGCDYSLFKDGIEP MWEDEKNKRGGRWLITLNKQ QRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKG DKIAIWTTECENREAVTHIG RVYKERLGLPPKIVIGYQSH ADTATKSGSTTKNRFVV
eIF4E binding region: KKQYDREFLLDFQFMPA

Data sets:

assigned_chemical_shifts
- set_1

Data type	Count
1H chemical shifts	197
13C chemical shifts	606
15N chemical shifts	197

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eif4e monomer	1
2	eIF4E binding region	2
3	7-methylguanosine diphosphate	3

Entities:

Entity 1, eif4e monomer 217 residues - 25097 Da.

1

MET

ALA

THR

VAL

GLU

PRO

GLU

THR

PRO

2

THR

PRO

ASN

PRO

THR

GLU

3

LYS

THR

GLU

SER

ASN

GLN

GLU

VAL

ALA

ASN

4

PRO

GLU

HIS

TYR

ILE

LYS

HIS

PRO

LEU

GLN

5

ASN

ARG

TRP

ALA

LEU

TRP

PHE

LYS

ASN

6

ASP

LYS

SER

LYS

THR

TRP

GLN

ALA

ASN

LEU

7

ARG

LEU

ILE

SER

LYS

PHE

ASP

THR

VAL

GLU

8

ASP

PHE

TRP

ALA

LEU

TYR

ASN

HIS

ILE

GLN

9

LEU

SER

ASN

LEU

MET

PRO

GLY

CYS

ASP

10

TYR

SER

LEU

PHE

LYS

ASP

GLY

ILE

GLU

PRO

11

MET

TRP

GLU

ASP

GLU

LYS

ASN

LYS

ARG

GLY

12

GLY

ARG

TRP

LEU

ILE

THR

LEU

ASN

LYS

GLN

13

GLN

ARG

SER

ASP

LEU

ASP

ARG

PHE

TRP

14

LEU

GLU

THR

LEU

CYS

LEU

ILE

GLY

GLU

15

SER

PHE

ASP

TYR

SER

ASP

VAL

CYS

16

GLY

ALA

VAL

ASN

VAL

ARG

ALA

LYS

GLY

17

ASP

LYS

ILE

ALA

ILE

TRP

THR

GLU

CYS

18

GLU

ASN

ARG

GLU

ALA

VAL

THR

HIS

ILE

GLY

19

ARG

VAL

TYR

LYS

GLU

ARG

LEU

GLY

LEU

PRO

20

PRO

LYS

ILE

VAL

ILE

GLY

TYR

GLN

SER

HIS

21

ALA

ASP

THR

ALA

THR

LYS

SER

GLY

SER

THR

22

THR

LYS

ASN

ARG

PHE

VAL

Entity 2, eIF4E binding region 17 residues - Formula weight is not available

1

LYS

GLN

TYR

ASP

ARG

GLU

PHE

LEU

2

ASP

PHE

GLN

PHE

MET

PRO

ALA

Entity 3, 7-methylguanosine diphosphate - C11 H19 N5 O11 P2 - 459.243 Da.

1

M7G

Samples:

sample_1: translation initiation factor 4E, [U-85% 2H;U-95% 13C; U-95% 15N], 0.8 mM; eIF4E binding region 0.8 mM; ammonium acetate 75 mM; ammonium sulfate 100 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.65 M

Experiments:

Name	Sample	Sample state	Sample conditions
TROSY-HNCO	sample_1	not available	Ex-cond_1
TROSY-HNCA	sample_1	not available	Ex-cond_1
TROSY-HN(CO)CA	sample_1	not available	Ex-cond_1
TROSY-HNCACB	sample_1	not available	Ex-cond_1
TROSY-HN(CO)CACB	sample_1	not available	Ex-cond_1
1H-15N-NOESY	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1EJH 1EJH
DBJ	BAD92356 BAE02210 BAG57978 BAE02210 BAG57978 BAD92356
GB	AAC02903 AAH94683 AAI36644 AAR32992 EAW94955
REF	NP_001075231 NP_001185730 NP_001185731 NP_003751 XP_002750436 XP_423296 XP_001165083 XP_001377349 NP_001075231 NP_003751
SP	O43432
SWISS-PROT	O43432
GenBank	EAW94955 EAW94956 AAH94683 AAR32992 AAC02903
EMBL	CAI14553 CAI12534 CAI12535 CAI12155 CAI12158
AlphaFold	O43432