BMRB Entry 5712

Title:
Backbone resonance assignment for human eIF4E in complex with m7GDP and a 17-amino acid peptide derived from human eIF4G
Deposition date:
2003-03-02
Original release date:
2006-05-30
Authors:
Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo
Citation:

Citation: Miura, Takaaki; Shiratori, Yasuhiko; Shimma, Nobuo. "Letter to the Editor: Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII "  J. Biomol. NMR 27, 279-280 (2003).

Assembly members:

Assembly members:
translation initiation factor 4E, polymer, 217 residues, 25097 Da.
eIF4E binding region, polymer, 17 residues, Formula weight is not available
M7G, non-polymer, 459.243 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts197
13C chemical shifts606
15N chemical shifts197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eif4e monomer1
2eIF4E binding region2
37-methylguanosine diphosphate3

Entities:

Entity 1, eif4e monomer 217 residues - 25097 Da.

1   METALATHRVALGLUPROGLUTHRTHRPRO
2   THRPROASNPROPROTHRTHRGLUGLUGLU
3   LYSTHRGLUSERASNGLNGLUVALALAASN
4   PROGLUHISTYRILELYSHISPROLEUGLN
5   ASNARGTRPALALEUTRPPHEPHELYSASN
6   ASPLYSSERLYSTHRTRPGLNALAASNLEU
7   ARGLEUILESERLYSPHEASPTHRVALGLU
8   ASPPHETRPALALEUTYRASNHISILEGLN
9   LEUSERSERASNLEUMETPROGLYCYSASP
10   TYRSERLEUPHELYSASPGLYILEGLUPRO
11   METTRPGLUASPGLULYSASNLYSARGGLY
12   GLYARGTRPLEUILETHRLEUASNLYSGLN
13   GLNARGARGSERASPLEUASPARGPHETRP
14   LEUGLUTHRLEULEUCYSLEUILEGLYGLU
15   SERPHEASPASPTYRSERASPASPVALCYS
16   GLYALAVALVALASNVALARGALALYSGLY
17   ASPLYSILEALAILETRPTHRTHRGLUCYS
18   GLUASNARGGLUALAVALTHRHISILEGLY
19   ARGVALTYRLYSGLUARGLEUGLYLEUPRO
20   PROLYSILEVALILEGLYTYRGLNSERHIS
21   ALAASPTHRALATHRLYSSERGLYSERTHR
22   THRLYSASNARGPHEVALVAL

Entity 2, eIF4E binding region 17 residues - Formula weight is not available

1   LYSLYSGLNTYRASPARGGLUPHELEULEU
2   ASPPHEGLNPHEMETPROALA

Entity 3, 7-methylguanosine diphosphate - C11 H19 N5 O11 P2 - 459.243 Da.

1   M7G

Samples:

sample_1: translation initiation factor 4E, [U-85% 2H;U-95% 13C; U-95% 15N], 0.8 mM; eIF4E binding region 0.8 mM; ammonium acetate 75 mM; ammonium sulfate 100 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.65 M

Experiments:

NameSampleSample stateSample conditions
TROSY-HNCOsample_1not availableEx-cond_1
TROSY-HNCAsample_1not availableEx-cond_1
TROSY-HN(CO)CAsample_1not availableEx-cond_1
TROSY-HNCACBsample_1not availableEx-cond_1
TROSY-HN(CO)CACBsample_1not availableEx-cond_1
1H-15N-NOESYsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAD92356 BAE02210 BAG57978 BAE02210 BAG57978 BAD92356
GB AAC02903 AAH94683 AAI36644 AAR32992 EAW94955
REF NP_001075231 NP_001185730 NP_001185731 NP_003751 XP_002750436 XP_423296 XP_001165083 XP_001377349 NP_001075231 NP_003751
SP O43432
SWISS-PROT O43432
GenBank EAW94955 EAW94956 AAH94683 AAR32992 AAC02903
EMBL CAI14553 CAI12534 CAI12535 CAI12155 CAI12158
AlphaFold O43432

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks