BMRB Entry 5711

Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR5711

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of HP0371, Biotin Carboxyl Carrier protein from Helocobacter pylori

Deposition date:

2003-03-01

Original release date:

2009-03-17

Authors:

Jung, Jinwon; Lee, Weontae

Citation:

Citation: Jung, Jinwon; Lee, Chul-Jin; Jeon, Young Ho; Cheong, Chaejoon; Lee, Weontae. "Solution structure and backbone dynamics of the biotinylation domain of Helicobacter pylori biotin-carboxyl carrier protein" B. Korean Chem. Soc. 29, 347-351 (2008).

Assembly members:

Assembly members:
Biotinoyl domain of HP0371, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Helicobater pylori Taxonomy ID: not available Superkingdom: Eubacteria Kingdom: not available Genus/species: Helicobater pylori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Biotinoyl domain of HP0371: GSVLSPMVGTFYHAPSPGAE PYVKAGDTLKKGQIVGIVEA MKIMNEIEVEYPCKVVSVEV GDAQPVEYGTKLIKVEKL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	211
15N chemical shifts	68
1H chemical shifts	440

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Biotinoyl domain of HP0371	1

Entities:

Entity 1, Biotinoyl domain of HP0371 78 residues - Formula weight is not available

1

GLY

SER

VAL

LEU

SER

PRO

MET

VAL

GLY

THR

2

PHE

TYR

HIS

ALA

PRO

SER

PRO

GLY

ALA

GLU

3

PRO

TYR

VAL

LYS

ALA

GLY

ASP

THR

LEU

LYS

4

LYS

GLY

GLN

ILE

VAL

GLY

ILE

VAL

GLU

ALA

5

MET

LYS

ILE

MET

ASN

GLU

ILE

GLU

VAL

GLU

6

TYR

PRO

CYS

LYS

VAL

SER

VAL

GLU

VAL

7

GLY

ASP

ALA

GLN

PRO

VAL

GLU

TYR

GLY

THR

8

LYS

LEU

ILE

LYS

VAL

GLU

LYS

LEU

Samples:

sample_15N: Biotinoyl domain of HP0371, [U-95% 15N], 2 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

sample_13C_15N: Biotinoyl domain of HP0371, [U-95% 13C; U-95% 15N], 0.7 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

sample_13C: Biotinoyl domain of HP0371, [U-95% 13C], 0.4 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM

Ex-cond_1: ionic strength: 0.3 M; pH: 7.0 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C CT-HSQC	not available	not available	not available
1H-15C TOCSY	not available	not available	not available
1H-15C NOESY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
1H-13C NOESY	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNHA	not available	not available	not available

Software:

NMRPipe v2.1 - processing of data

SPARKY v3.106 - peak assignment, peak picking

AutoAssign v1.7 - automated backbond assignment

CYANA v1.0.6 - structure calculation with automated NOESY assignment

NMR spectrometers:

Bruker DRX 500 MHz
Varian INOVA-UNITY-Plus 500 MHz

DBJ	BAJ55623 BAJ56401 BAJ58603 BAJ60120 BAM96952
EMBL	CAX29567 CBI65976
GB	AAD07435 ABF85088 ACD48521 ACI27779 ACJ08202
REF	NP_207169 WP_001053746 WP_001053747 WP_001053748 WP_001053749