BMRB Entry 5705

Name: 1H, 13C and 15N resonance assignment of an RNA UUCG Tetraloop
Published: 2004-02-11

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PDB ID: 2koc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5705
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of an RNA UUCG Tetraloop

Deposition date:

2003-02-21

Original release date:

2004-02-11

Authors:

Furtig, Boris; Richter, Christian; Bermel, Wolfgang; Schwalbe, Harald; Nozinovic, Senada; Jonker, Hendrik

Citation:

Citation: Furtig, Boris; Richter, Christian; Bermel, Wolfgang; Schwalbe, Harald. "New NMR experiments for RNA nucleobase resonance assignment and chemical shift analysis of an RNA UUCG tetraloop" J. Biomol. NMR 28, 69-79 (2004).
PubMed: 14739640

Assembly members:

Assembly members:
uucg tetraloop, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
uucg tetraloop: GGCACUUCGGUGCC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	132
15N chemical shifts	47
1H chemical shifts	140
31P chemical shifts	14

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	uucg tetraloop, uucg	1

Entities:

Entity 1, uucg tetraloop, uucg 14 residues - Formula weight is not available

1

G

C

A

C

U

C

G

2

U

G

C

Samples:

sample_1: uucg tetraloop, [U-13C; U-15N], 0.7 mM

sample_conditions_1: pH: 6.4; temperature: 298 K

sample_conditions_2: pH: 6.4; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz
Bruker AV 700 MHz
Bruker AV 800 MHz