BMRB Entry 5704

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5704

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N assignment of mth677 protein from Methanobacterium Thermoautotrophicum

Deposition date:

2003-02-17

Original release date:

2003-06-09

Authors:

Blanco, Francisco; Campos-Olivas, Ramon; Yee, Adelinda; Arrowsmith, Cheryl; Rico, Manuel

Citation:

Citation: Blanco, Francisco; Campos-Olivas, Ramon; Yee, Adelinda; Arrowsmith, Cheryl; Rico, Manuel. "1H, 15N and 13C resonance assignment of the hypothetical protein mth677from Mthanobacterium thermoautotrophicum" .

Assembly members:

Assembly members:
mth677, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanobacterium thermoautotrophicum Taxonomy ID: 187420 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanothermobacter thermoautotrophicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mth677: GSHMSLRKLTEGDLDEISSF LHNTISDFILKRVSAKEIVD IDITVLVEYTDELKVDISAE LYLDELSDADPGIVDEAVDA AYRSLESFLDGFRE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	414
15N chemical shifts	93
1H chemical shifts	617

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mth677	1

Entities:

Entity 1, mth677 94 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

LEU

ARG

LYS

LEU

THR

2

GLU

GLY

ASP

LEU

ASP

GLU

ILE

SER

PHE

3

LEU

HIS

ASN

THR

ILE

SER

ASP

PHE

ILE

LEU

4

LYS

ARG

VAL

SER

ALA

LYS

GLU

ILE

VAL

ASP

5

ILE

ASP

ILE

THR

VAL

LEU

VAL

GLU

TYR

THR

6

ASP

GLU

LEU

LYS

VAL

ASP

ILE

SER

ALA

GLU

7

LEU

TYR

LEU

ASP

GLU

LEU

SER

ASP

ALA

ASP

8

PRO

GLY

ILE

VAL

ASP

GLU

ALA

VAL

ASP

ALA

9

ALA

TYR

ARG

SER

LEU

GLU

SER

PHE

LEU

ASP

10

GLY

PHE

ARG

GLU

Samples:

sample_1: mth677, [U-15N], 1.2 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; H2O 90 % v/v; D2O 10 % v/v; Zinc chloride 20 uM

sample_2: mth677, [U-15N; U-13C], 1 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; H2O 90 % v/v; D2O 10 % v/v; Zinc chloride 20 uM

sample_3: mth677, [U-15N; U-13C], 1 mM; Sodium phosphate 25 mM; Sodim chloride 450 mM; DTT 10 mM; benzamidine 1 mM; D2O 100 % v/v; Zinc chloride 20 uM

condition_1: pH*: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

xwinnmr v3.1, Bruker - acquisition, plotting, processing

NMRPipe v97.027.12.56, . - data processing

NMRDraw v2.2, . - processing and analysis, spectral visualization

NMRView v5.0.3, . - spectra analysis, spectra visualization

CYANA v1.0.5, . - structure calculation

NMR spectrometers:

unknown unknown 0 MHz

PDB	1PU1
DBJ	BAM69854
GB	AAB85182
REF	NP_275819 WP_010876315
SP	O26773
AlphaFold	O26773