BMRB Entry 5697

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5697

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N resonance assignments of the PDZ domain of ZASP in complex with the EF hand domains of alpha-actinin-2

Deposition date:

2003-02-17

Original release date:

2004-04-07

Authors:

Au, Yunghan; Atkinson, R.; Faulkner, Georgine; Frenkiel, Thomas; Joseph, Catherine; Kelly, Geoff; Muskett, Frederick; Pallavicini, Alberto; Pastore, Annalisa

Citation:

Citation: Au, Yunghan; Atkinson, R.; Guerrini, R.; Kelly, Geoff; Joseph, Catherine; Martin, S.; Muskett, Frederick; Pallavicini, Alberto; Faulkner, Georgine; Pastore, Annalisa. "Solution Structure of ZASP PDZ Domain; Implications for Sarcomere Ultrastructure and Enigma Family Redundancy" Structure (Cambridge, MA, U. S.) 12, 611-622 (2004).
PubMed: 15062084

Assembly members:

Assembly members:
Z-band alternatively spliced PDZ motif protein, polymer, 85 residues, 9139.40 Da.
Alpha-Actinin-2 EF-hand domains, polymer, 154 residues, 17163.2 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Z-band alternatively spliced PDZ motif protein: MAYSVTLTGPGPWGFRLQGG KDFNMPLTISRITPGSKAAQ SQLSQGDLVVAIDGVNTDTM THLEAQNKIKSASYNLSLTL QKSKR
Alpha-Actinin-2 EF-hand domains: GAMGRDAKGITQEQMNEFRA SFNHFDRRKNGLMDHEDFRA CLISMGYDLGEAEFARIMTL VDPNGQGTVTFQSFIDFMTR EPADTDTAEQVIASFRILAS DKPYILAEELRRELPPDQAQ YCIKRMPAYSGPGSVPGALD YAAFSSALYGESDL

Data sets:

assigned_chemical_shifts
- Assignment_1

Data type	Count
1H chemical shifts	434
15N chemical shifts	82

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZASP-PDZ Domain	1
2	Alpha-Actinin-2 EF-hand domain	2

Entities:

Entity 1, ZASP-PDZ Domain 85 residues - 9139.40 Da.

1

MET

ALA

TYR

SER

VAL

THR

LEU

THR

GLY

PRO

2

GLY

PRO

TRP

GLY

PHE

ARG

LEU

GLN

GLY

3

LYS

ASP

PHE

ASN

MET

PRO

LEU

THR

ILE

SER

4

ARG

ILE

THR

PRO

GLY

SER

LYS

ALA

GLN

5

SER

GLN

LEU

SER

GLN

GLY

ASP

LEU

VAL

6

ALA

ILE

ASP

GLY

VAL

ASN

THR

ASP

THR

MET

7

THR

HIS

LEU

GLU

ALA

GLN

ASN

LYS

ILE

LYS

8

SER

ALA

SER

TYR

ASN

LEU

SER

LEU

THR

LEU

9

GLN

LYS

SER

LYS

ARG

Entity 2, Alpha-Actinin-2 EF-hand domain 154 residues - 17163.2 Da.

1

GLY

ALA

MET

GLY

ARG

ASP

ALA

LYS

GLY

ILE

2

THR

GLN

GLU

GLN

MET

ASN

GLU

PHE

ARG

ALA

3

SER

PHE

ASN

HIS

PHE

ASP

ARG

LYS

ASN

4

GLY

LEU

MET

ASP

HIS

GLU

ASP

PHE

ARG

ALA

5

CYS

LEU

ILE

SER

MET

GLY

TYR

ASP

LEU

GLY

6

GLU

ALA

GLU

PHE

ALA

ARG

ILE

MET

THR

LEU

7

VAL

ASP

PRO

ASN

GLY

GLN

GLY

THR

VAL

THR

8

PHE

GLN

SER

PHE

ILE

ASP

PHE

MET

THR

ARG

9

GLU

PRO

ALA

ASP

THR

ASP

THR

ALA

GLU

GLN

10

VAL

ILE

ALA

SER

PHE

ARG

ILE

LEU

ALA

SER

11

ASP

LYS

PRO

TYR

ILE

LEU

ALA

GLU

LEU

12

ARG

GLU

LEU

PRO

ASP

GLN

ALA

GLN

13

TYR

CYS

ILE

LYS

ARG

MET

PRO

ALA

TYR

SER

14

GLY

PRO

GLY

SER

VAL

PRO

GLY

ALA

LEU

ASP

15

TYR

ALA

PHE

SER

ALA

LEU

TYR

GLY

16

GLU

SER

ASP

LEU

Samples:

Sample_1: Z-band alternatively spliced PDZ motif protein, [U-15N], 0.14 mM; Alpha-Actinin-2 EF-hand domains 0.28 mM; sodium phosphate 20 mM; sodium azide 0.2 % v/v; deuterium oxide 10 % v/v

Condition_1: pH: 6.6; temperature: 300 K; ionic strength: 0.02 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available

Software:

nmrPipe - processing

XEASY - analysis

NMR spectrometers:

Varian UnityPlus 500 MHz
Varian UnityPlus 600 MHz

PDB	4D1E
DBJ	BAD92758 BAG37672 BAH11921 BAH12587 BAH12632 BAD92758 BAG37672
EMBL	CAB61269 CAI13778 CAB61269 CAH73201
GB	AAA51583 AAH47901 AAH51770 AAI02909 AIC48214
REF	NP_001029807 NP_001094 NP_001230595 NP_001265272 NP_001265273 NP_001094
SP	P35609 Q3ZC55
TPG	DAA14356
SWISS-PROT	P35609
GenBank	EAW70064 EAW70065 AAH47901 AAH51770 AAA51583
AlphaFold	P35609 Q3ZC55