BMRB Entry 5692
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5692
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Velyvis, Algirdas; Vaynberg, Julie; Yang, Yanwu; Vinogradova, Olga; Zhang, Yongjun; Wu, Chuanyue; Qin, Jun. "Structural and Functional Insights into PINCH LIM4 Domain-mediated Integrin
Signaling" Nat. Struct. Biol. 10, 558-564 (2003).
PubMed: 12794636
Assembly members:
4th LIM domain of PINCH protein, polymer, 66 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
4th LIM domain of PINCH protein: GSMGVPICGACRRPIEGRVV
NAMGKQWHVEHFVCAKCEKP
FLGHRHYERKGLAYCETHYN
QLFGDV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 227 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 446 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LIM4 | 1 |
| 2 | Zinc ion (II) | 2 |
Entities:
Entity 1, LIM4 66 residues - Formula weight is not available
| 1 | GLY | SER | MET | GLY | VAL | PRO | ILE | CYS | GLY | ALA | ||||
| 2 | CYS | ARG | ARG | PRO | ILE | GLU | GLY | ARG | VAL | VAL | ||||
| 3 | ASN | ALA | MET | GLY | LYS | GLN | TRP | HIS | VAL | GLU | ||||
| 4 | HIS | PHE | VAL | CYS | ALA | LYS | CYS | GLU | LYS | PRO | ||||
| 5 | PHE | LEU | GLY | HIS | ARG | HIS | TYR | GLU | ARG | LYS | ||||
| 6 | GLY | LEU | ALA | TYR | CYS | GLU | THR | HIS | TYR | ASN | ||||
| 7 | GLN | LEU | PHE | GLY | ASP | VAL |
Entity 2, Zinc ion (II) - Zn - 65.409 Da.
| 1 | ZN |
Samples:
the_sample: 4th LIM domain of PINCH protein, [U-95% 13C; U-95% 15N], 1.7 mM; NaCl 50 mM; Na2HPO4 25 mM
the_conditions: ionic strength: 125 mM; pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | the_sample | not available | the_conditions |
| HNCACB | the_sample | not available | the_conditions |
| HNCO | the_sample | not available | the_conditions |
| N,C-edited-NOESY | the_sample | not available | the_conditions |
| HNHA | the_sample | not available | the_conditions |
| HCCH-TOCSY | the_sample | not available | the_conditions |
Software:
NMRPipe -
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| GB | AGW21640 EFB20618 ELW62207 KFP50847 KFP56517 |
| REF | XP_002832613 XP_002930391 XP_004005972 XP_004938580 XP_005953270 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks