BMRB Entry 5690

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5690
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for Cobra Neurotoxin II
Deposition date:
2003-02-11
Original release date:
2003-05-08
Authors:
Bocharov, Eduard; Arseniev, Alexandre
Citation:

Citation: Lyukmanova, Ekaterina; Bocharov, Eduard; Ermolyuk, Yaroslav; Schulga, Alexey; Pluzhnikov, Kirill; Dolgikh, Dmitry; Arseniev, Alexander; Kirpichnikov, Mikhail. "Bacterial expession of isotope labeled snake neurotoxin, disulfide-rich protein"  .

Assembly members:

Assembly members:
neurotoxin II, polymer, 61 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Central Asian cobra   Taxonomy ID: 8657   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Naja oxiana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
neurotoxin II: LECHNQQSSQPPTTKTCSGE TNCYKKWWSDHRGTIIERGC GCPKVKPGVNLNCCRTDRCN N

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts400
13C chemical shifts254
15N chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1neurotoxin II, nt21

Entities:

Entity 1, neurotoxin II, nt2 61 residues - Formula weight is not available

1   LEUGLUCYSHISASNGLNGLNSERSERGLN
2   PROPROTHRTHRLYSTHRCYSSERGLYGLU
3   THRASNCYSTYRLYSLYSTRPTRPSERASP
4   HISARGGLYTHRILEILEGLUARGGLYCYS
5   GLYCYSPROLYSVALLYSPROGLYVALASN
6   LEUASNCYSCYSARGTHRASPARGCYSASN
7   ASN

Samples:

sample_1: neurotoxin II, [U-95% 13C; U-95% 15N], 2 mM

Ex-cond_1: pH: 3.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableEx-cond_1
13C-HSQCsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1

Software:

XEASY v1.2.11 - peak assignments

NMR spectrometers:

  • VARIAN UNITY 600 MHz

Related Database Links:

BMRB 16060 19704 5052
PDB
SP P01427 P59276
AlphaFold P01427 P59276 P01427 P59276

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks