BMRB Entry 5679
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5679
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Hemmi, Hikaru; Kuno, Atsushi; Ito, Shigeyasu; Kaneko, Satoshi; Hasegawa, Tsunemi. "Letter to the Editor: 1H, 13C and 15N Chemical Shift Assignment of Xylan-binding
Domain from Streptomyces olivaceoviridis E-86 Beta-xylanase " J. Biomol. NMR 27, 91-92 (2003).
PubMed: 12878846
Assembly members:
xylan-binding domain, polymer, 132 residues, Formula weight is not available
Natural source: Common Name: Streptomyces olivaceoviridis Taxonomy ID: 1921 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces olivaceoviridis
Experimental source: Production method: recombinant technology Host organism: Esherichia coli Vector: pET27
Entity Sequences (FASTA):
xylan-binding domain: STPPPSGGGQIKGVGSGRCL
DVPNASTTDGTQVQLYDCHS
ATNQQWTYTDAGELRVYGDK
CLDAAGTGNGTKVQIYSCWG
GDNQKWRLNSDGSIVGVQSG
LCLDAVGGGTANGTLIQLYS
CSNGSNQRWTRT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 808 |
| 13C chemical shifts | 515 |
| 15N chemical shifts | 151 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | xbd monomer | 1 |
Entities:
Entity 1, xbd monomer 132 residues - Formula weight is not available
| 1 | SER | THR | PRO | PRO | PRO | SER | GLY | GLY | GLY | GLN | ||||
| 2 | ILE | LYS | GLY | VAL | GLY | SER | GLY | ARG | CYS | LEU | ||||
| 3 | ASP | VAL | PRO | ASN | ALA | SER | THR | THR | ASP | GLY | ||||
| 4 | THR | GLN | VAL | GLN | LEU | TYR | ASP | CYS | HIS | SER | ||||
| 5 | ALA | THR | ASN | GLN | GLN | TRP | THR | TYR | THR | ASP | ||||
| 6 | ALA | GLY | GLU | LEU | ARG | VAL | TYR | GLY | ASP | LYS | ||||
| 7 | CYS | LEU | ASP | ALA | ALA | GLY | THR | GLY | ASN | GLY | ||||
| 8 | THR | LYS | VAL | GLN | ILE | TYR | SER | CYS | TRP | GLY | ||||
| 9 | GLY | ASP | ASN | GLN | LYS | TRP | ARG | LEU | ASN | SER | ||||
| 10 | ASP | GLY | SER | ILE | VAL | GLY | VAL | GLN | SER | GLY | ||||
| 11 | LEU | CYS | LEU | ASP | ALA | VAL | GLY | GLY | GLY | THR | ||||
| 12 | ALA | ASN | GLY | THR | LEU | ILE | GLN | LEU | TYR | SER | ||||
| 13 | CYS | SER | ASN | GLY | SER | ASN | GLN | ARG | TRP | THR | ||||
| 14 | ARG | THR |
Samples:
sample_1: xylan-binding domain, [U-95% 13C; U-90% 15N], mM
Ex-cond_1: pH: 6.1 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCOCAHACACONH | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CCONH | not available | not available | not available |
| HCCONH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-NOESY | not available | not available | not available |
| 1H-TOCSY | not available | not available | not available |
| CT-13C-HSQC | not available | not available | not available |
| NOESY-CT-HSQC | not available | not available | not available |
| TOCSY-CT-HSQC | not available | not available | not available |
Software:
XWINNMR v2.6 - acquisition
FELIX v2000 - processing
SPARKY v3.1 - peak assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks