BMRB Entry 5677

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5677
MolProbity Validation Chart

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Title:
1H, 13C, and 15N assignment of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase
Deposition date:
2003-01-24
Original release date:
2004-03-15
Authors:
Prutzman, Kirk; Gao, Guanghua; Schaller, Michael; Campbell, Sharon
Citation:

Citation: Gao, Guanghua; Prutzman, Kirk; King, M; Scheswohl, D; DeRose, E; London, R; Schaller, Michael; Campbell, Sharon. "NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide: evidence for a two-site binding model."  J. Biol. Chem. 279, 8441-8451 (2004).
PubMed: 14662767

Assembly members:

Assembly members:
focal adhesion targeting domain, polymer, 146 residues, 15878 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
focal adhesion targeting domain: GSPGISGGGGGIRSNDKVYE NVTGLVKAVIEMSSKIQPAP PEEYVPMVKEVGLALRTLLA TVDESLPVLPASTHREIEMA QKLLNSDLAELINKMKLAQQ YVMTSLQQEYKKQMLTAAHA LAVDAKNLLDVIDQARLKMI SQSRPH

Data sets:
Data typeCount
1H chemical shifts803
13C chemical shifts560
15N chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FAT1

Entities:

Entity 1, FAT 146 residues - 15878 Da.

1   GLYSERPROGLYILESERGLYGLYGLYGLY
2   GLYILEARGSERASNASPLYSVALTYRGLU
3   ASNVALTHRGLYLEUVALLYSALAVALILE
4   GLUMETSERSERLYSILEGLNPROALAPRO
5   PROGLUGLUTYRVALPROMETVALLYSGLU
6   VALGLYLEUALALEUARGTHRLEULEUALA
7   THRVALASPGLUSERLEUPROVALLEUPRO
8   ALASERTHRHISARGGLUILEGLUMETALA
9   GLNLYSLEULEUASNSERASPLEUALAGLU
10   LEUILEASNLYSMETLYSLEUALAGLNGLN
11   TYRVALMETTHRSERLEUGLNGLNGLUTYR
12   LYSLYSGLNMETLEUTHRALAALAHISALA
13   LEUALAVALASPALALYSASNLEULEUASP
14   VALILEASPGLNALAARGLEULYSMETILE
15   SERGLNSERARGPROHIS

Samples:

Sample_1: focal adhesion targeting domain, [U-13C; U-15N], 0.85 mM

FAT-cond: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions

Software:

NMRview v5.0.4 -

NMRPipe -

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5266 5924
PDB
GB AAA48765 AAA48773 EMC80181 EOA99770 KFM11460
REF NP_990766 XP_002191290 XP_004174254 XP_005021656 XP_005021657
SP Q00944
AlphaFold Q00944 Q00944 Q00944

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