BMRB Entry 5676
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5676
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: M'Barek, S.; Mosbah, A.; Sandoz, G.; Fajloun, Z.; Olamendi-Portugal, T.; Rochat, H.; Sampieri, F.; Guijarro, J.; Mansuelle, P.; Delepierre, M.; De Waard, M.; Sabatier, J.. "Synthesis and Characterization of Pi4, a Scorpion Toxin from Pandinus
imperator that acts on K+ Channels" Eur. J. Biochem. 270, 3583-3592 (2003).
PubMed: 12919322
Assembly members:
Pi4 scorpion toxin, polymer, 38 residues, 4180 Da.
Natural source: Common Name: Scorpion Taxonomy ID: 55084 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Pandinus imperator
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Pi4 scorpion toxin: IEAIRCGGSRDCYRPCQKRT
GCPNAKCINKTCKCYGCS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 446 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pi4 scorpion toxin | 1 |
Entities:
Entity 1, Pi4 scorpion toxin 38 residues - 4180 Da.
| 1 | ILE | GLU | ALA | ILE | ARG | CYS | GLY | GLY | SER | ARG | ||||
| 2 | ASP | CYS | TYR | ARG | PRO | CYS | GLN | LYS | ARG | THR | ||||
| 3 | GLY | CYS | PRO | ASN | ALA | LYS | CYS | ILE | ASN | LYS | ||||
| 4 | THR | CYS | LYS | CYS | TYR | GLY | CYS | SER |
Samples:
sample_1: Pi4 scorpion toxin 2.5 mM; sodium acetate 5 mM; D2O 10%
sample_2: Pi4 scorpion toxin 3.2 mM; sodium acetate 5 mM; D2O 100%
sample_cond_1: ionic strength: 5 mM; pH: 4.0; pressure: 1 atm; temperature: 303 K
sample_cond_2: ionic strength: 5 mM; pH*: 4.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| P-COSY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
VNMR v6.1C - collection, raw spectral data processing
NMRView v5.03 - data analysis
NMR spectrometers:
- Varian INOVA 500 MHz