BMRB Entry 5667

Title:
Solution structure of the first zinc-finger domain from ZNF265
Deposition date:
2003-01-23
Original release date:
2003-08-07
Authors:
Plambeck, C.; Kwan, A.; Adams, D.; Westman, B.; van der Weyden, L; Medcalf, R; Morris, B.; Mackay, J.
Citation:

Citation: Plambeck, C.; Kwan, A.; Adams, D.; Westman, B.; van der Weyden, L.; Medcalf, R.; Morris, B.; Mackay, J.. "The Structure of the Zinc Finger Domain from Human Splicing Factor ZNF265 Fold"  J. Biol. Chem. 278, 22805-22811 (2003).
PubMed: 12657633

Assembly members:

Assembly members:
First zinc finger of Zinc finger protein 265, polymer, 45 residues, 4995.7 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4t-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
First zinc finger of Zinc finger protein 265: GSMSTKNFRVSDGDWICPDK KCGNVNFARRTSCDRCGREK TTGPI

Data sets:
Data typeCount
1H chemical shifts245

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1znf265-F1 monomer1
2ZINC ION (II)2

Entities:

Entity 1, znf265-F1 monomer 45 residues - 4995.7 Da.

1   GLYSERMETSERTHRLYSASNPHEARGVAL
2   SERASPGLYASPTRPILECYSPROASPLYS
3   LYSCYSGLYASNVALASNPHEALAARGARG
4   THRSERCYSASPARGCYSGLYARGGLULYS
5   THRTHRGLYPROILE

Entity 2, ZINC ION (II) - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: First zinc finger of Zinc finger protein 265 1 mM; TCEP 2 mM; ZnZO4 1.7 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
NOESY Short mixing timesample_1not availablesample_cond_1
TOCSY Short mixing timesample_1not availablesample_cond_1

Software:

xwinnmr v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.2 - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB