BMRB Entry 5667

Name: Solution structure of the first zinc-finger domain from ZNF265
Published: 2003-08-07

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PDB ID: 1n0z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5667
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the first zinc-finger domain from ZNF265

Deposition date:

2003-01-23

Original release date:

2003-08-07

Authors:

Plambeck, C.; Kwan, A.; Adams, D.; Westman, B.; van der Weyden, L; Medcalf, R; Morris, B.; Mackay, J.

Citation:

Citation: Plambeck, C.; Kwan, A.; Adams, D.; Westman, B.; van der Weyden, L.; Medcalf, R.; Morris, B.; Mackay, J.. "The Structure of the Zinc Finger Domain from Human Splicing Factor ZNF265 Fold" J. Biol. Chem. 278, 22805-22811 (2003).
PubMed: 12657633

Assembly members:

Assembly members:
First zinc finger of Zinc finger protein 265, polymer, 45 residues, 4995.7 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4t-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
First zinc finger of Zinc finger protein 265: GSMSTKNFRVSDGDWICPDK KCGNVNFARRTSCDRCGREK TTGPI

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	245

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	znf265-F1 monomer	1
2	ZINC ION (II)	2

Entities:

Entity 1, znf265-F1 monomer 45 residues - 4995.7 Da.

1

GLY

SER

MET

SER

THR

LYS

ASN

PHE

ARG

VAL

2

SER

ASP

GLY

ASP

TRP

ILE

CYS

PRO

ASP

LYS

3

LYS

CYS

GLY

ASN

VAL

ASN

PHE

ALA

ARG

4

THR

SER

CYS

ASP

ARG

CYS

GLY

ARG

GLU

LYS

5

THR

GLY

PRO

ILE

Entity 2, ZINC ION (II) - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: First zinc finger of Zinc finger protein 265 1 mM; TCEP 2 mM; ZnZO4 1.7 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	not available	sample_cond_1
2D TOCSY	sample_1	not available	sample_cond_1
DQF-COSY	sample_1	not available	sample_cond_1
NOESY Short mixing time	sample_1	not available	sample_cond_1
TOCSY Short mixing time	sample_1	not available	sample_cond_1

Software:

xwinnmr v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.2 - structure solution

NMR spectrometers:

Bruker DRX 600 MHz