BMRB Entry 5665

Title:
ClpX Zinc Binding Domain
Deposition date:
2003-01-21
Original release date:
2004-03-15
Authors:
Donaldson, Logan
Citation:

Citation: Donaldson, Logan; Wojtyra, Urszula; Houry, Walid. "Solution structure of the dimeric zinc binding domain of the chaperone ClpX."  J. Biol. Chem. 278, 48991-48996 (2003).
PubMed: 14525985

Assembly members:

Assembly members:
ClpX Zinc Finger, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ClpX Zinc Finger: TDKRKDGSGKLLYCSFCGKS QHEVRKLIAGPSVYICDECV DLCNDIIREEIKEVAPHR

Data sets:
Data typeCount
1H chemical shifts320
13C chemical shifts203
15N chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ClpX Zinc Finger, monomer 11
2ClpX Zinc Finger, monomer 21

Entities:

Entity 1, ClpX Zinc Finger, monomer 1 58 residues - Formula weight is not available

1   THRASPLYSARGLYSASPGLYSERGLYLYS
2   LEULEUTYRCYSSERPHECYSGLYLYSSER
3   GLNHISGLUVALARGLYSLEUILEALAGLY
4   PROSERVALTYRILECYSASPGLUCYSVAL
5   ASPLEUCYSASNASPILEILEARGGLUGLU
6   ILELYSGLUVALALAPROHISARG

Samples:

sample_1: ClpX Zinc Finger, [U-15N; U-13C], 1.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; sodium chloride 150 mM

standard_conditions: pH: 7.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N HSQC-NOESYnot availablenot availablenot available
13C HSQC-NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C(CC)-TOCSY-(CO)NHnot availablenot availablenot available
H(CC)-TOCSY-(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
15N HSQCnot availablenot availablenot available
13C-filtered NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAA94669 BAB33915 BAE73947 BAE76218 BAG75988
EMBL CAA80816 CAD08908 CAG74059 CAH20200 CAL13168
GB AAA16116 AAB40194 AAC45783 AAC73541 AAG54788
PIR AD0558
REF NP_286180 NP_308519 NP_414972 NP_455046 NP_459445
SP A1A8A7 A1JNN1 A4TPE2 A4W7A9 A6T5I1
AlphaFold A1A8A7 A1JNN1 A4TPE2 A4W7A9 A6T5I1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks