BMRB Entry 5665
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5665
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Citation: Donaldson, Logan; Wojtyra, Urszula; Houry, Walid. "Solution structure of the dimeric zinc binding domain
of the chaperone ClpX." J. Biol. Chem. 278, 48991-48996 (2003).
PubMed: 14525985
Assembly members:
ClpX Zinc Finger, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
ClpX Zinc Finger: TDKRKDGSGKLLYCSFCGKS
QHEVRKLIAGPSVYICDECV
DLCNDIIREEIKEVAPHR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 320 |
| 13C chemical shifts | 203 |
| 15N chemical shifts | 47 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ClpX Zinc Finger, monomer 1 | 1 |
| 2 | ClpX Zinc Finger, monomer 2 | 1 |
Entities:
Entity 1, ClpX Zinc Finger, monomer 1 58 residues - Formula weight is not available
| 1 | THR | ASP | LYS | ARG | LYS | ASP | GLY | SER | GLY | LYS | ||||
| 2 | LEU | LEU | TYR | CYS | SER | PHE | CYS | GLY | LYS | SER | ||||
| 3 | GLN | HIS | GLU | VAL | ARG | LYS | LEU | ILE | ALA | GLY | ||||
| 4 | PRO | SER | VAL | TYR | ILE | CYS | ASP | GLU | CYS | VAL | ||||
| 5 | ASP | LEU | CYS | ASN | ASP | ILE | ILE | ARG | GLU | GLU | ||||
| 6 | ILE | LYS | GLU | VAL | ALA | PRO | HIS | ARG |
Samples:
sample_1: ClpX Zinc Finger, [U-15N; U-13C], 1.5 mM; sodium phosphate 20 mM; sodium azide 0.02%; sodium chloride 150 mM
standard_conditions: pH: 7.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC-NOESY | not available | not available | not available |
| 13C HSQC-NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| C(CC)-TOCSY-(CO)NH | not available | not available | not available |
| H(CC)-TOCSY-(CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| 15N HSQC | not available | not available | not available |
| 13C-filtered NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA94669 BAB33915 BAE73947 BAE76218 BAG75988 |
| EMBL | CAA80816 CAD08908 CAG74059 CAH20200 CAL13168 |
| GB | AAA16116 AAB40194 AAC45783 AAC73541 AAG54788 |
| PIR | AD0558 |
| REF | NP_286180 NP_308519 NP_414972 NP_455046 NP_459445 |
| SP | A1A8A7 A1JNN1 A4TPE2 A4W7A9 A6T5I1 |
| AlphaFold | A1A8A7 A1JNN1 A4TPE2 A4W7A9 A6T5I1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks