BMRB Entry 5664
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5664
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Findlow, Stuart; Winsor, Claire; Simpson, Thomas; Crosby, John; Crump, Matthew. "Solution Structure and Dynamics of Oxytetracycline Polyketide Synthase
Acyl Carrier Protein from Streptomyces rimosus " Biochemistry 42, 8423-8433 (2003).
PubMed: 12859187
Assembly members:
Acyl Carrier Protein, polymer, 95 residues, 9916 Da.
Natural source: Common Name: soil bacterium Taxonomy ID: 1927 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces rimosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT-7
Entity Sequences (FASTA):
Acyl Carrier Protein: MTLLTLSDLLTLLRECAGEE
ESIDLGGDVEDVAFDALGYD
SLALLNTVGRIERDYGVQLG
DDAVEKATTPRALIEMTNAS
LTGASPSAGGAARDK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 585 |
| 15N chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | otc ACP | 1 |
Entities:
Entity 1, otc ACP 95 residues - 9916 Da.
| 1 | MET | THR | LEU | LEU | THR | LEU | SER | ASP | LEU | LEU | ||||
| 2 | THR | LEU | LEU | ARG | GLU | CYS | ALA | GLY | GLU | GLU | ||||
| 3 | GLU | SER | ILE | ASP | LEU | GLY | GLY | ASP | VAL | GLU | ||||
| 4 | ASP | VAL | ALA | PHE | ASP | ALA | LEU | GLY | TYR | ASP | ||||
| 5 | SER | LEU | ALA | LEU | LEU | ASN | THR | VAL | GLY | ARG | ||||
| 6 | ILE | GLU | ARG | ASP | TYR | GLY | VAL | GLN | LEU | GLY | ||||
| 7 | ASP | ASP | ALA | VAL | GLU | LYS | ALA | THR | THR | PRO | ||||
| 8 | ARG | ALA | LEU | ILE | GLU | MET | THR | ASN | ALA | SER | ||||
| 9 | LEU | THR | GLY | ALA | SER | PRO | SER | ALA | GLY | GLY | ||||
| 10 | ALA | ALA | ARG | ASP | LYS |
Samples:
sample_1: Acyl Carrier Protein, [U-97% 15N], 1 mM
Ex-cond_1: pH: 5.3 na; temperature: 298 K; ionic strength: 0.015 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
NMRPipe - NMR data processing
Pipp - NMR data assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA80986 |
| GB | AAA03334 AAZ78327 ELQ83294 KEF12048 |
| REF | WP_003981021 WP_030372734 |
| SP | P43677 |
| AlphaFold | P43677 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks