BMRB Entry 5663

Title:
Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation site threonine
Deposition date:
2003-01-16
Original release date:
2004-03-15
Authors:
Shin-ya, Ohki
Citation:

Citation: Ohki, S.; Eto, M.; Shimizu, M.; Takada, R.; Brautigan, D.; Kainosho, M.. "Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threonine residue."  J. Mol. Biol. 326, 1539-1547 (2003).
PubMed: 12595264

Assembly members:

Assembly members:
CPI-17 mutant T38D, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts100
1H chemical shifts594

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CPI-17 22-120 T38D1

Entities:

Entity 1, CPI-17 22-120 T38D 99 residues - Formula weight is not available

1   GLYPROGLYGLYSERPROGLYGLYLEUGLN
2   LYSARGHISALAARGVALASPVALLYSTYR
3   ASPARGARGGLULEUGLNARGARGLEUASP
4   VALGLULYSTRPILEASPGLYARGLEUGLU
5   GLULEUTYRARGGLYARGGLUALAASPMET
6   PROASPGLUVALASNILEASPGLULEULEU
7   GLULEUGLUSERGLUGLUGLUARGSERARG
8   LYSILEGLNGLYLEULEULYSSERCYSTHR
9   ASNPROTHRGLUASNPHEVALGLNGLULEU
10   LEUVALLYSLEUARGGLYLEUHISLYS

Samples:

sample_1: CPI-17 mutant T38D, [U-13C; U-15N], 1.0 mM

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 5175 5662
PDB
DBJ BAA22995
REF NP_001179999 NP_999502 XP_004015712 XP_004330765 XP_006215130
SP O18734
TPG DAA19818
AlphaFold O18734

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks